The principle of the µMACS™ and MultiMACS™ Protein A/G Kits is based on superparamagnetic MicroBeads conjugated with protein A or protein G. For (co-)immunoprecipitation experiments the cell lysates may be incubated with monoclonal or polyclonal antibodies or with a wide range of antibody sources such as hybridoma supernatant, ascites, or serum. This step is followed by magnetic labeling with Protein A or Protein G MicroBeads. The protein-antibody-Protein A/G MicroBeads complex is then loaded onto a µMACS Column placed in the magnetic field of a µMACS Separator. The magnetically labeled complex and associated proteins are retained on the column during the washing steps and can be eluted and analyzed by SDS-PAGE. The µMACS and MultiMACS Protein A/G Kits are also suitable for chromatin immunoprecipitation (ChIP) studies. In contrast to the standard ChIP protocol this streamlined protocol saves 90% of laboratory time and allows for working with just 106 cells. Please click here to download the protocol Chromatin immunoprecipitation (ChIP). To see an application note reported by David Mulholland and colleagues from the Prostate Research Center, Vancouver, Canada click here. The µMACS Protein A/G Kit was developed for manual, low-throughput applications in combination with the µMACS Separator. The procedure can easily be up-scaled with the MultiMACS Protein A/G Kits to a semi- or fully automated, flexible and parallel processing of 8–96 samples by utilizing the MultiMACS M96/M96thermo Separators.
Download Protein Research Brochure