Products and Services - MACSmolecular - Protein research - Epitope-tagged protein isolation and detection - Anti-c-myc antibodies

Anti-c-myc antibodies

  • Fast: avoid the secondary antibody step in Western blot applications
  • Convenient: direct detection after primary antibody incubation
  • Versatile: available as FITC and biotin conjugates for (in)direct fluorescent labeling of c-myc fusion proteins

Overview

The monoclonal Anti-c-myc antibody facilitates the fast and convenient detection of c-myc-tagged fusion proteins, expressed in either prokaryotic or eukaryotic cells. The antibody is available conjugated with horseradish peroxidase (HRP), FITC, or biotin for fast and convenient analysis of c-myc-tagged fusion proteins. Conjugation of the Anti-c-myc antibody to HRP simplifies Western blot or ELISA analysis as the incubation with secondary antibodies becomes dispensable. After incubation the Anti-c-myc-HRP antibody can be directly detected using commercially available chemiluminescent reagents.

Details

Applications

The monoclonal Anti-c-myc antibody specifically recognizes proteins that are tagged with the c-myc epitope (EQKLISEEDL). The Anti-c-myc-HRP antibody enables direct protein detection in Western blot and ELISA analyses without the need for a secondary antibody. Anti-c-myc antibodies conjugated to FITC enable direct flow cytometry and fluorescence microscopy analyses while indirect fluorescent labeling of c-myc fusion protein expressing cells is possible with the biotin-conjugated antibody.

Gallery

Figure 1

Immunofluorescence analysis of c-myc-BDCA-2 expressing CHO cells. CHO cells transiently expressing c-myc-BDCA-2 were analyzed by fluorescence microscopy 24 hours after transfection. A) Double staining of c-myc-BDCA-2 with Anti-c-myc-FITC and Anti-BDCA-2-Biotin/Anti-Biotin-Alexa 546. B) Double staining of c-myc-BDCA-2 with Anti-c-myc-Biotin/Anti-Biotin-Alexa 546 and BDCA-2-FITC. Red: Alexa 546-labeling, green: FITC-labeling, yellow: FITC-Alexa 546 overlay.
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Brochures and posters

Protein research [PDF, 2.6 MB, EN]

Product Order no. Price

Anti-c-myc-FITC

Capacity: for 30 tests (1)

Capacity

(1)One test corresponds to the analysis of 1×106 total cells.
 
Data sheet
130-099-616 $85.00

Anti-c-myc-FITC

Capacity: for 100 tests (1)

Capacity

(1)One test corresponds to the analysis of 1×106 total cells.
 
Data sheet
130-092-472 $225.00

Anti-c-myc-Biotin

Capacity: for 30 tests (1)

Capacity

(1)One test corresponds to the analysis of 1×106 total cells.
 
Data sheet
130-099-373 $85.00

Anti-c-myc-Biotin

Capacity: for 100 tests (1)

Capacity

(1)One test corresponds to the analysis of 1×106 total cells.
 
Data sheet
130-092-471 $225.00

Anti-c-myc-HRP

Content: 100 µL
 
Data sheet
130-092-113 $345.00

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Miltenyi Biotec Inc.
Phone: +1 800 FOR MACS
Fax:+1 877 591 1060
macs@miltenyibiotec.com
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