The monoclonal Anti-c-myc antibody facilitates the fast and convenient detection of c-myc-tagged fusion proteins, expressed in either prokaryotic or eukaryotic cells. The antibody is available conjugated with horseradish peroxidase (HRP), FITC, or biotin for fast and convenient analysis of c-myc-tagged fusion proteins. Conjugation of the Anti-c-myc antibody to HRP simplifies Western blot or ELISA analysis as the incubation with secondary antibodies becomes dispensable. After incubation the Anti-c-myc-HRP antibody can be directly detected using commercially available chemiluminescent reagents.
The monoclonal Anti-c-myc antibody specifically recognizes proteins that are tagged with the c-myc epitope (EQKLISEEDL). The Anti-c-myc-HRP antibody enables direct protein detection in Western blot and ELISA analyses without the need for a secondary antibody. Anti-c-myc antibodies conjugated to FITC enable direct flow cytometry and fluorescence microscopy analyses while indirect fluorescent labeling of c-myc fusion protein expressing cells is possible with the biotin-conjugated antibody.
Immunofluorescence analysis of c-myc-BDCA-2 expressing CHO cells. CHO cells transiently expressing c-myc-BDCA-2 were analyzed by fluorescence microscopy 24 hours after transfection. A) Double staining of c-myc-BDCA-2 with Anti-c-myc-FITC and Anti-BDCA-2-Biotin/Anti-Biotin-Alexa 546. B) Double staining of c-myc-BDCA-2 with Anti-c-myc-Biotin/Anti-Biotin-Alexa 546 and BDCA-2-FITC. Red: Alexa 546-labeling, green: FITC-labeling, yellow: FITC-Alexa 546 overlay.