Products and Services - MACSmolecular - Protein research - Epitope-tagged protein isolation and detection

Fast analytical-scale epitope-tagged protein isolation and detection

  • Fast: from cell lysis to pure protein in just 1.5 hours
  • Convenient: avoid multiple centrifugation and buffer removal steps
  • Sensitive: enables working with limited starting material

The group of products for the isolation of epitope-tagged proteins consists of µMACS and MultiMACS™ Isolation Kits for the most common epitopes, i.e., GFP, HA, c-myc, GST, His, and DYKDDDDK (also known as FLAG®-tag). The isolation principle is based on superparamagnetic MicroBeads conjugated to the respective epitope-specific antibodies. The MicroBeads are incubated with the cell lysate and bind instantly to the target protein. The sample is loaded onto a µMACS Column placed in the magnetic field of a µMACS Separator. The magnetically labeled epitope-tagged and associated proteins are retained on the column during the washing steps and can be eluted either under native or denaturing conditions. The µMACS Epitope Tag Isolation Kits were developed for manual, low-throughput applications in combination with the µMACS Separator. The procedure can easily be up-scaled with the MultiMACS Epitope Tag Isolation Kits to a semi- or fully automated, flexible processing of 8–96 samples in parallel by utilizing the MultiMACS M96/M96thermo Separators.


Following elution the GFP-, HA-, c-myc-, His-, and DYKDDDDK-tagged fusion proteins can be detected using Miltenyi Biotec’s monoclonal antibodies that are specific for these epitopes. All antibodies are available conjugated with horseradish peroxidase for Western blot or ELISA applications. In addition HA, c-myc, His, and DYKDDDDK antibodies are coupled to fluorescent dyes (i.e. FITC, PE) for direct analysis using fast and convenient flow cytometry and fluorescent microscopy. Biotin-conjugated antibodies are also available for indirect fluorescent labeling.


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62 products available
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Product Order no. Price

Anti-c-myc-FITC

Capacity: for 30 tests (1)

Capacity

(1)One test corresponds to the analysis of 1×106 total cells.
 
130-099-616 $85.00

Anti-c-myc-FITC

Capacity: for 100 tests (1)

Capacity

(1)One test corresponds to the analysis of 1×106 total cells.
 
130-092-472 $225.00

Anti-c-myc-Biotin

Capacity: for 30 tests (1)

Capacity

(1)One test corresponds to the analysis of 1×106 total cells.
 
130-099-373 $85.00

Anti-c-myc-Biotin

Capacity: for 100 tests (1)

Capacity

(1)One test corresponds to the analysis of 1×106 total cells.
 
130-092-471 $225.00

Anti-c-myc-HRP

Content: 100 µL
 
130-092-113 $345.00

Anti-DYKDDDDK-Vio515

Capacity: for 30 tests (1)

Capacity

(1)One test corresponds to the analysis of 1×106 total cells.
 
130-109-756 $135.00

Anti-DYKDDDDK-Vio515

Capacity: for 100 tests (1)

Capacity

(1)One test corresponds to the analysis of 1×106 total cells.
 
130-109-709 $345.00

Anti-DYKDDDDK-FITC

Capacity: for 30 tests (1)

Capacity

(1)One test corresponds to the analysis of 1×106 total cells.
 
130-101-571 $85.00

Anti-DYKDDDDK-FITC

Capacity: for 100 tests (1)

Capacity

(1)One test corresponds to the analysis of 1×106 total cells.
 
130-101-570 $240.00

Anti-DYKDDDDK-PE

Capacity: for 30 tests (1)

Capacity

(1)One test corresponds to the analysis of 1×106 total cells.
 
130-101-577 $100.00
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Showing 1-10 of 62


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Miltenyi Biotec Inc.
Phone: +1 800 FOR MACS
Fax:+1 877 591 1060
macs@miltenyibiotec.com
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