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Brain Tumor Dissociation Kit

Overview

The Brain Tumor Dissociation Kits have been developed specifically for the gentle and effective tissue dissociation of human and mouse brain tumors, such as glioblastoma multiforme (GBM) or medulloblastoma. Since tumor tissue can be sticky it is recommended to use the kit in combination with the gentleMACS™ Dissociator or the gentleMACS Octo Dissociator. In less than an hour, the tissue dissociation procedure efficiently yields high numbers of viable cells. Dissociated cells are immediately ready for further applications such as cell culture, flow cytometry, molecular applications, or for cell isolation using MACS® Technology.

Details

Background information

The quality of starting material is important for any experiment, and a perfect single-cell suspension is a prerequisite for many downstream applications. Glioblastoma, neuroblastoma, medulloblastoma, xenograft brain tumor samples can be effectively dissociated into single-cell suspensions using the Brain Tumor Dissociation Kit1 and a gentleMACS™ Dissociator.
The Brain Tumor Dissociation Kits (T) and (P) are based on trypsin and papain, respectively. Some antigen epitopes are degraded by papain, others by trypsin. The kit based on papain is best suited for use with CD133 MicroBeads, CD11b (Microglia) MicroBeads and Anti-A2B5 MicroBeads, whereas, for example, the Anti-GLAST (ACSA-1) MicroBead Kit requires the use of the trypsin-based kit.
Please see Figure 1 in the gallery for further information with regard to epitope sensitivity and kit selection. In general, a papain-based protocol gives by far the highest yield of viable cells.
Myelin Removal Beads II are recommended to effectively deplete myelin debris from the resulting single cell suspension for determination of the cell numbers and improved downstream applications like magnetic cell isolation.

Gallery

Figure 1

Figure 1
Antigen compatibility of Dissociation Kits. For many applications, including MACS Cell Separation, it is crucial to conserve the integrity of particular cell surface epitopes during tissue dissociation. The table shows which kits conserve which specific epitopes. Epitope sensitivities have been tested using Miltenyi Biotec's antibodies if available.
Antigen compatibility of Dissociation Kits. For many applications, including MACS Cell Separation, it is crucial to conserve the integrity of particular cell surface epitopes during tissue dissociation. The table shows which kits conserve which specific epitopes. Epitope sensitivities have been tested using Miltenyi Biotec's antibodies if available.

Miltenyi TV

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How to isolate pure viable microglia

How to isolate pure viable microglia

Watch this hands-on protocol showing you how to isolate microglial cells from adult mouse brain. The Miltenyi Biotec protocol is faster, standardized, reproducible, and easy to scale up.

Related Items

Library

Selected references

  1. Környei et al. (2007) Astroglia-derived retinoic acid is a key factor in glia-induced neurogenesis. FASEB J. 21: 2496–2509.
  2. Santagata, S. et al. et al. (2014) Intraoperative mass spectrometry mapping of an onco-metabolite to guide brain tumor surgery. Proc. Natl. Acad. Sci. U.S.A. 111: 11121–11126.
  3. Skog, J. et al. (2008) Glioblastoma microvesicles transport RNA and proteins that promote tumour growth and provide diagnostic biomarkers. Nat. Cell Biol. 10(12): 1470–1476.
  4. Kijima, N. et al. (2012) CD166/Activated leukocyte cell adhesion molecule is expressed on glioblastoma progenitor cells and involved in the regulation of tumor cell invasion. Neuro-oncology 14: 1254–1264.
  5. Janiszewska, M. et al. (2012) Imp2 controls oxidative phosphorylation and is crucial for preserving glioblastoma cancer stem cells. Genes Dev. 26: 1926–1944.
  6. Piao, Y. et al. (2012) Glioblastoma resistance to anti-VEGF therapy is associated with myeloid cell infiltration, stem cell accumulation, and a mesenchymal phenotype. Neuro-oncology 14: 1379–1392.
  7. Golebiewska, A. et al. (2013) Side population in human glioblastoma is non-tumorigenic and characterizes brain endothelial cells. Brain 136: 1462–1475.
  8. Piao, Y. et al. (2013) Acquired resistance to anti-VEGF therapy in glioblastoma is associated with a mesenchymal transition. Clin. Cancer Res. 19: 4392–4403.
  9. Zeiner, P. S. et al. (2014) MIF receptor CD74 is restricted to microglia/macrophages, associated with a M1-polarized immune milieu and prolonged patient survival in gliomas. Brain Pathol. 25: 491–504.

Brochures and posters

Neuroscience research brochure [PDF, 1.9 MB, EN]

Scientific posters

Product Order no. Price

Brain Tumor Dissociation Kit (P)

Capacity: for 25 preparations
 
Data sheet
130-095-942 $450.00

Brain Tumor Dissociation Kit (T)

Capacity: for 25 preparations
 
Data sheet
130-095-939 $450.00

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