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Anti-PTK7 (CCK-4) antibodies, human (clone: 188B)

Overview

The Anti-PTK7 (CCK-4) antibody (clone 188B) recognizes human protein tyrosine kinase-7 (PTK7). PTK7, also known as colon carcinoma kinase-4 (CCK-4), is a receptor protein tyrosine kinase (RPTK)-like molecule that contains a catalytically inactive tyrosine kinase domain.1,2 PTK7 was identified to be important for neural development as a regulator of planar cell polarity, being required for convergent extension movement and neural tube closure.3 Using the Anti-PTK7 (CCK-4) antibody, PTK7 expression was recently analyzed on blood and bone marrow cells. PTK7 was shown to be expressed on plasmacytoid dendritic cells, CD141 (BDCA-3)high type-2 myeloid dendritic cells, and CD34+ hematopoietic progenitor cells (HPCs). PTK7 was detected on early (CD34+ CD133+) and late (CD34+ CD133) HPCs. In tonsils, PTK7 was also found on some T cells.

Technical data


Clone 188B
Isotype mouse IgG2a
Isotype control Mouse IgG2a – isotype control antibodies
Alternative names of antigen
Entrez Gene ID 5754
Molecular mass of antigen [kDa] 115
Cross-reactivity
Distribution of antigen
Endotoxin level
Product format Antibodies are supplied in buffer containing stabilizer and 0.05% sodium azide.
Fixation Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
Storage Store protected from light at 2–8 °C. Do not freeze.
Available conjugates VioBright FITC, PE, APC, Biotin, pure

Gallery

Figure 1

Human PBMCs were stained with Anti-PTK7-APC and CD303 (BDCA-2)-FITC, CD141 (BDCA-3)-PE, or CD34-FITC, respectively. Cells were analyzed by flow cytometry. Plasmacytoid dendritic cells (PDCs) are identified by CD303 (BDCA-2), type-2 myeloid dendritic cells (MDC2s) by CD141 (BDCA-3), and hematopoietic stem cells (HPCs) by CD34 expression.
Identification of PTK7+ CD303 (BDCA-2)+ PDCs
Identification of PTK7+ CD141 (BDCA-3)high MDC2s
Identification of PTK7+ CD34+ HPCs

Related Items

Library

Selected references

  1. Mossie et al. (1995) Colon carcinoma kinase-4 defines a new subclass of the receptor tyrosine kinase family. Oncogene 11: 2179–2184.
  2. Park et al. (1996) Characterization of the human full-length PTK7 cDNA encoding a receptor protein tyrosine kinase-like molecule closely related to chick KLG. J. Biochem. 119: 235–239.
  3. Lu et al. (2004) PTK7/CCK-4 is a novel regulator of planar cell polarity in … Nature 430: 93–98.
  4. Katoh, M. et al. (2007) Comparative integromics on non-canonical WNT or planar cell polarity signaling molecules: transcriptional mechanism of PTK7 in colorectal cancer and that of SEMA6A in undifferentiated ES cells. Int. J. Mol. Med. 20: 405–409.

Product Order no. Price

Anti-PTK7 (CCK-4)-VioBright FITC, human (clone: 188B)

For research use only
Capacity: for 30 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-104-771 $125.00

Anti-PTK7 (CCK-4)-VioBright FITC, human (clone: 188B)

For research use only
Capacity: for 100 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-104-724 $305.00

Anti-PTK7 (CCK-4)-PE, human (clone: 188B)

For research use only
Capacity: for 30 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-099-109 $90.00

Anti-PTK7 (CCK-4)-PE, human (clone: 188B)

For research use only
Capacity: for 100 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-091-364 $255.00

Anti-PTK7 (CCK-4)-APC, human (clone: 188B)

For research use only
Capacity: for 30 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-099-660 $115.00

Anti-PTK7 (CCK-4)-APC, human (clone: 188B)

For research use only
Capacity: for 100 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-091-366 $275.00

Anti-PTK7 (CCK-4)-Biotin, human (clone: 188B)

For research use only
Capacity: for 30 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-100-176 $75.00

Anti-PTK7 (CCK-4)-Biotin, human (clone: 188B)

For research use only
Capacity: for 100 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-091-365 $215.00

Anti-PTK7 (CCK-4) pure, human (clone: 188B)

For research use only
Content: 100 µg in 1 mL

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-091-578 $120.00

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Miltenyi Biotec Inc.
Phone: +1 800 FOR MACS
Fax:+1 530 745 2806
macs@miltenyibiotec.com
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