Products and Services - MACS Flow Cytometry - Reagents - Kits and assays - 7-Color Immunophenotyping Kit, human

7-Color Immunophenotyping Kit, human

Overview

The 7-Color Immunophenotyping Kit simplifies the flow cytometric evaluation of cell fractions for automated and manual immunofluorescent staining. The kit cocktail has been designed for the reliable identification of human monocytes, neutrophils, eosinophils, and T, B, and NK lymphocyte populations as well as CD4+, CD8+, and CD56+CD3+ T cell subsets in human blood.

Details

Applications

Evaluation of leukocyte subsets in PBMCs, whole blood, or lysed whole blood.

Gallery

Figure 1

Whole blood from a healthy donor was stained with the 7-Color Immunophenotyping Kit, human. Staining was carried out at 4 °C for 10 minutes. Subsequently, red blood cells were lysed by incubation using 1× Red Blood Cell Lysis Solution at room temperature for 15 minutes and analyzed by flow cytometry using the Express Mode of the MACSQuant® Analyzer.
As a preliminary step for elimination of doublets a gate around single cells in forward scatter area (FSC-A) versus forward scatter height (FSC-H) was set (A). To identify the major circulating blood cell types CD45 was used to target all leukocytes (B). Theses cells were further separated from debris via forward scatter (FSC) and side scatter (SSC) (C). CD19 has been used to define B cells and CD3 for T cells (D). The T cells were separated in CD4+ and CD8+ T cells (E). CD14/SSC intermediate were selected to identify monocytes (F). Monocytes were excluded and the remaining cells were further characterized. A gate was defined on CD16/SSChigh cells to identify mature neutrophils, which could be separated from eosinophils due to the absence of CD16 expression. A CD16/CD56/SSClow gate was used for sub-characterization (G). These cells were further characterized by gating on CD3/CD56+ T cells and CD56/CD16+ NK cells (H).
A:
B:
C:
D:
E:
F:
G:
H:
Product Order no. Price

7-Color Immunophenotyping Kit, human

Capacity: for 50 tests
 
Data sheet
130-098-456 $850.00

Customer login

Go

Forgot password?
Register for account

0 Shopping cart

Contact

Miltenyi Biotec Inc.
Phone: +1 800 FOR MACS
Fax:+1 877 591 1060
macs@miltenyibiotec.com
Contact overview

Product finder

Antibody panel builder

Toolbox

Loading...
New MACS® Antibody Format

New MACS® Antibody Format

Antibody Panel Builder

Antibody Panel Builder

Get rewarded for the reagents you love

Get rewarded for reagents

Change country

Please select your country to continue