Products and Services - MACS Flow Cytometry - Reagents - Antibodies and dyes - Vio Dye Antibody Conjugates

Vio® Dye Antibody Conjugates

  • Bright: superior mean fluorescence intensity for excellent signals
  • Distinct: high stain index for clear population discrimination
  • Hassle-free: ideal for multicolor experiments due to low compensation

Figure 1: Emission spectra of VioDyes: VioBlue, VioBright 515, Vio515, VioGreen, VioBright FITC, PE-Vio615, PerCP-Vio700, PE-Vio770, APC-Vio770

Violet Laser (405 nm)

VioBlue® Dye (Emmax 452 nm)
VioBlue is Coumarin-based dye with excitation and emission wavelengths of 400 nm and 455 nm, respectively. As a superior alternative to Pacific Blue™, Alexa Fluor® 405, or BD™ Horizon™ V450, multiplexing of VioBlue with other fluorochromes is easily possible, adding to the variety of marker combination for multiparameter flow cytometry.
VioGreen™ Dye (Emmax 520 nm)
VioGreen is a large stokes shift fluorophore, emitting strong fluorescence at 520 nm upon excitation at 405 nm. It is a non-protein fluorophore with significantly increased mean fluorescence intensities and higher stain indices than comparable fluorochromes such as Pacific Orange™, Krome Orange™, and BD Horizon™ V500.
 

Blue Laser (488 nm)

VioBright™ 515 (Emmax 514 nm)
VioBright 515 is an extraordinary bright dye for the FITC-channel. The proprietary multimerization technology allows a 4 fold increase in brightness over the traditional FITC dye. The dye is excited by the blue laser (488 nm) and it´s emission peak is at 514 nm. Improved brightness in combination with 25% less spill-over into the PE-channel allows an optimal detection of rare surface markers in the FITC channel.
Vio®515 (Emmax 514 nm)
Vio515 is a dye for the FITC-channel. Improved brightness over FITC allows the analysis of intracellular markers that were not available in this channel before, thus giving more flexibility in multicolor panel design. In combination with 25% less spill-over into the PE-channel Vio515 allows an optimal detection of intracellular markers, such as cytokines and transcription factors. The dye is excited by the blue laser (488 nm) and it´s emission peak is at 514 nm.
VioBright™ FITC (Emmax 522 nm)
VioBright™ FITC is a blue laser (488 nm) excited revolutionary dye, which allows an increased number of FITC molecules per antibody, as compared to conventional FITC conjugation. With brightness similar to PE, VioBright FITC expands the dimensions of multicolor flow analysis. In addition, it provides a bright alternative for confident detection of rare cells, as well as dim and uncharacterized markers.
PE-Vio®615 (Emmax 619 nm)
PE-Vio®615 is a tandem dye with PE as the donor dye and Vio® 615 as the acceptor dye. This tandem dye is optimized for efficient donor-to-acceptor dye energy transfer, high fluorescent intensity, and low spillover into the donor dye detection channel. Designed to be a superior alternative to ECD, PE-Texas Red®, PE-efluor® 610, PE-CF594 and PE/Dazzle™ 594, this Vio Dye expands the options for flexible multicolor panel design and provides a bright dye for confident detection of dim and rare markers.
PerCP-Vio®700 (Emmax 704 nm)
The PerCP-Vio700 Dye is a tandem conjugate that combines the peridinin chlorophyll protein (PerCP) and the new Vio700 Dye to emit a strong fluorescence at 655–730 nm upon blue laser excitation at 488 nm. This dye is suited perfectly for the B3 channel of the MACSQuant® Analyzer.
PE-Vio®770 (Emmax 770 nm)
The PE-Vio®770 Dye is a tandem conjugate, like PE-Cy™7, that exploits the principle of fluorescence-resonance-energy-transfer (FRET) allowing for large Stokes shifts between the absorbed energy of a fluorescence donor (PE) and the emission wavelength of a suitable acceptor (Vio770) dye.

Yellow Laser (561 nm)

PE-Vio® 615 (Emmax 619 nm)
PE-Vio® 615 is a tandem dye with PE as the donor dye and Vio® 615 as the acceptor dye. This tandem dye is optimized for efficient donor-to-acceptor dye energy transfer, high fluorescent intensity, and low spillover into the donor dye detection channel. Designed to be a superior alternative to ECD, PE-Texas Red®, PE-efluor® 610, PE-CF594 and PE/Dazzle™ 594, this Vio Dye expands the options for flexible multicolor panel design and provides a bright dye for confident detection of dim and rare markers.
PE-Vio770™ (Emmax 770 nm)
The PE-Vio770™ Dye is a tandem conjugate, like PE-Cy™7, that exploits the principle of fluorescence-resonance-energy-transfer (FRET) allowing for large Stokes shifts between the absorbed energy of a fluorescence donor (PE) and the emission wavelength of a suitable acceptor (Vio770) dye.

Red Laser (635 nm)

APC-Vio770™ (Emmax 770 nm)
The APC-Vio770™ Dye is a tandem conjugate, like APC-Cy™7 or APC-H7, that exploits the principle of fluorescence-resonance-energy transfer (FRET) allowing for large Stokes shifts between the absorbed energy of a fluorescence donor (APC) and the emission wavelength of a suitable acceptor (Vio770).

Excitation and emission data of the Vio Dyes

Fluorochrome Excitation Exmax(nm) Emmax(nm) MACSQuant Analyzer MACSQuant VYB
  laser (nm)     Channel Filter (nm) Channel Filter (nm)
VioBlue 405 400 452 V1 450/50 V1 450/50
VioGreen 405 388 520 V2 525/50 V2 525/50
VioBright 515 488 488 514 B1 525/50 B1 525/50
Vio 515 488 488 514 B1 525/50 B1 525/50
VioBright FITC 488 496 522 B1 525/50 B1 525/50
FITC 488 495 520 B1 525/50 B1 525/50
PE 488 or 561 565 578 B2 585/40 Y1 586/15
PE-Vio615 488 or 561 565 619 B3 655-730 Y2 615/20
PerCP 488 482 675 B3 655-730 N/A N/A
PerCP-Vio700 488 482 704 B3 655-730 N/A N/A
PE-Vio770 488 or 561 565 775 B4 750 LP Y4 750 LP
APC 561 or 635 652 660 R1 655-730 Y3 661/20
APC-Vio770 561 or 635 652 775 R2 750 LP Y4 750 PL

Support Material

  • Fluorescence spectra overview poster
  • Fluorochromes and instruments poster
  • Fluorochrome brightness index
  • Quality assured – from lot to lot
  • Tandem Signal Enhancer
  • Vio® Dyes technical specifications
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    Miltenyi Biotec Inc.
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