Products and Services - MACS Flow Cytometry - Reagents - Antibodies and dyes - REAfinity Antibodies

Recombinant antibodies for flow cytometry

  • High purity and lot-to-lot consistency for greater reproducibility
  • Eliminates tedious and costly Fc receptor-blocking steps
  • One universal isotype control for convenience and cost savings

REAfinity™ Antibodies are recombinant antibodies that provide superior lot-to-lot consistency and purity compared to mouse or rat monoclonal antibodies. They've been recombinantly engineered to produce highly specific antibodies that require no FcR blocking step. Additionally, they all have the same IgG1 isotype.

 

Flow cytometry is in their genes 

 

We back up our claims that REAfinity™ Antibodies are the best antibodies available with real data. They are demonstrated to be highly specific, result in better staining than mouse or rat monoclonals, and without the need of Fcγ receptor blocking step.

  • Highly specific: better staining than mouse and rat monoclonals
  • Clear resolution of target population: enables straightforward data analysis
  • High stain index: due to an optimized fluorochrome labeling process and low background

Figure 1: Specific detection of CD158b2+ cells with REA147. PBMCs were stained with a PE-conjugated REAfinity Antibody (A) and mouse monoclonal PE-conjugated antibody (B) recognizing CD158b2. Additionally, staining with CD56-APC (# 130-100-698) was performed followed by flow cytometry analysis on the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.

Figure 2: Specific recognition of CD116+ cells with REA211.  PBMCs were stained with a PE-conjugated
REAfinity Antibody (A) and mouse monoclonal PE-conjugated antibody (B) recognizing CD116. Additionally, staining with CD14-FITC (# 130-080-701) was performed followed by flow cytometry analysis on the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.

Figure 3: Staining with REAfinity Antibodies shows no background signal, even without FcR block. Human PBMCs were stained with either a mouse monoclonal CD158a-PE antibody or REAfinity CD158a-PE (clone:REA284). Staining was performed without (top) and with (bottom) FcR block. The mouse monoclonal antibody binds unspecifically to CD56-negative cells (A). Staining with the REAfinity Antibody, shows no background signal Cells were analyzed by flow cytometry on the MACSQuant® Analyzer 10. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.

Flexibility in flow cytometry with thousands of recombinant antibodies

REAfinity clones are available for hundreds of specificities. Search our list for your antibody of interest:

All Reafinity Clones

They are conjugated to a variety of fluorochromes to address multicolor flow cytometry needs. Current options include traditional dyes, like FITC, PE, APC, and Miltenyi Biotec´s proprietary Vio® Dyes:

Figure 1: Emission spectra of VioDyes: VioBlue, VioBright 515, Vio515, VioGreen, VioBright FITC, PE-Vio615, PerCP-Vio700, PE-Vio770, APC-Vio770

One universal isotype control

One universal isotype control

REAfinity™ Antibodies all have human IgG1 as their isotype. The result is that you need only one type of isotype control for all REA clones, named REA Control Antibodies or clone REA293. This control is available in two formats – one for surface- and one for intracellular-expressed antigens.

What does one isotype control per fluorochrome mean to you?
  • You will experience significant cost savings since you will need only one control.
  • You will save time spent on searching for the antibody isotype.

High purity - no Ig chain mixtures

Mouse monoclonal antibodies often contain mixtures of different IgG heavy and light-antibody chains (figure 6). This impurity can lead to lot-lot variations and could impact the reproducibility of your experiments. 

REAfinity™ Antibodies offer several major advantages over mouse monoclonal antibodies for long term studies, including:

  1. REAfinity Antibody products contain only contain one heavy and one light-chain for higher lot-to-lot consistency (figure 6).
  2. The unmatched quality of REAfinity Antibodies also arises from its manufacture in mammalian cells and production under a highly controlled manufacturing process.
  3. Compared to the average mouse monoclonal antibody, REAfinity Antibodies offer greater purity and higher lot-to-lot consistency to make REAfinity Antibodies your reagent of choice.

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Miltenyi Biotec Inc.
Phone: +1 800 FOR MACS
Fax:+1 877 591 1060
macs@miltenyibiotec.com
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