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CD203c antibodies, human (clone: FR3-16A11)

Overview

Clone FR3-16A11 recognizes CD203c, a glycosylated type II transmembrane molecule that belongs to the family of ecto-nucleotide pyrophosphatase/ phosphodiesterase (E-NPP3) enzymes. Among hematopoietic cells, expression of CD203c is restricted to basophils, mast cells and their precursors, and has been described as specific for this lineage. Protein and/or mRNA expression of CD203c has also been found in solid tissues such as uterus or prostate. Basophils and mast cells are key producers of mediators that drive the onset of inflammatory responses, e.g. in allergy. Allergen challenge leads to a rapid up-regulation of activation markers such as CD203c or CD63. Due to its restricted expression pattern, CD203c is discussed as a specific marker to monitor the allergen-induced activation of basophils, e.g. in flow cytometric basophil activation tests of the peripheral blood.

Technical data


Antigen CD203c
Clone FR3-16A11
Isotype mouse IgG1κ
Isotype control Mouse IgG1 – isotype control antibodies
Alternative names of antigen ENPP3, B10, NPP3, PD-IBETA, PDNP3, E-NPP3, PD-1b
Entrez Gene ID 5169
Molecular mass of antigen [kDa] 100
Cross-reactivity
Distribution of antigen basophils, liver, mast cells, myeloid cells, pancreas, small intestine
Endotoxin level
Product format Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
Fixation Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
Storage Store protected from light at 2–8 °C. Do not freeze.
Available conjugates PE, APC, PE-Vio770, Biotin, pure

Gallery

Figure 1

Human peripheral blood mononuclear cells (PBMCs) were stained with CD203c antibodies as well as with CD123 antibodies and analyzed by flow cytometry. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. For all other conjugates the FcR Blocking Reagent has been used to avoid Fc receptor–mediated antibody labeling. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandems.

Related Items

Library

Selected references

  1. Bühring, H. J. et al. (1999) The monoclonal antibody 97A6 defines a novel surface antigen expressed on human basophils and their multipotent and unipotent progenitors. Blood 94: 2343–2356.
  2. Goding, J. W. (2000) Ecto-enzymes: physiology meets pathology. J. Leukoc. Biol. 67: 285–311.
  3. Bühring, H. J. et al. (2004) The basophil-specific ectoenzyme E-NPP3 (CD203c) as a marker for cell activation and allergy diagnosis. Int. Arch. Allergy Immunol. 133: 317–329.
  4. Kahlert, H. et al. (2003) Measurement of basophil-activating capacity of grass pollen allergens, allergoids Clin. Exp. Allergy 33: 1266–1272.
  5. Hauswirth, A. W. et al. (2002) Recombinant allergens promote expression of CD203c on basophils in sensitized individuals. J. Allergy Clin. Immunol. 110: 102–109.

Product Order no. Price

CD203c-PE, human (clone: FR3-16A11)

For research use only
Capacity: for 30 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-099-238 price on request here

CD203c-PE, human (clone: FR3-16A11)

For research use only
Capacity: for 100 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-092-343 price on request here

CD203c-APC, human (clone: FR3-16A11)

For research use only
Capacity: for 30 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-098-888 price on request here

CD203c-APC, human (clone: FR3-16A11)

For research use only
Capacity: for 100 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-092-344 price on request here

CD203c-PE-Vio770, human (clone: FR3-16A11)

For research use only
Capacity: for 30 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-101-556 price on request here

CD203c-PE-Vio770, human (clone: FR3-16A11)

For research use only
Capacity: for 100 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-101-583 price on request here

CD203c-Biotin, human (clone: FR3-16A11)

For research use only
Capacity: for 100 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-092-345 price on request here

CD203c pure, human (clone: FR3-16A11)

For research use only
Content: 100 µg in 1 mL
 
Data sheet
130-092-392 price on request here

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Miltenyi Biotec Inc.
Phone: +1 800 FOR MACS
Fax:+1 877 591 1060
macs@miltenyibiotec.com
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