Products and Services - MACS Flow Cytometry - Reagents - Antibodies and dyes - CD158i (KIR2DS4) antibodies, human

CD158i (KIR2DS4) antibodies, human (clone: JJC11.6)

Overview

Clone JJC11.6 recognizes CD158i (KIR2DS4), a member of the killer immunoglobulin-like receptor (KIR) family expressed on natural killer (NK) cells. CD158i (KIR2DS4) provides an activation signal for NK lytic activity upon interaction with its ligand, HLA-Cw4, in an antigen-independent manner.
KIRs are monomeric receptors possessing high allelic polymorphism with either 2 or 3 Ig-like extracellular domains. The receptor family can be subdivided functionally according to the length of their cytoplasmic tail in inhibitory and activating KIRs.

Technical data


Antigen CD158i (KIR2DS4)
Clone JJC11.6
Isotype mouse IgG1κ
Isotype control Mouse IgG1 – isotype control antibodies
Alternative names of antigen KIR2DS4
Entrez Gene ID 3809
Molecular mass of antigen [kDa] 31
Cross-reactivity
Distribution of antigen NK cells, T cells
Endotoxin level
Product format Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
Fixation Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
Storage Store protected from light at 2–8 °C. Do not freeze.
Available conjugates PE, APC, PE-Vio770, Biotin, pure

Gallery

Figure 1

Human peripheral blood mononuclear cells (PBMCs) were stained with CD158i (KIR2DS4) antibodies as well as with CD56 antibodies and analyzed by flow cytometry using the MACSQuant® Analyzer. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandems.

Related Items

Library

Selected references

  1. Kratz, G. et al. (2004) MHC Class I-independent recognition of NK-activating receptor KIR2DS4. J. Immunol. 173: 1819–1825.
  2. Djeu, J. et al. (2002) A view to a kill: signals triggering cytotoxicity. Clin. Cancer Res. 8: 636–640.
  3. Hsu, K. et al. (2002) The killer cell immunoglobin-like (KIR) genomic region: gene-order, haplotypes and allelic polymorphism. Immunol. Rev. 190: 40–52.
  4. Selvakumar, A. et al. (1997) Polymorphism and domain variability of human killer cell inhibitory receptors. Immunol. Rev. 155: 183–196.

Product Order no. Price

CD158i (KIR2DS4)-PE, human (clone: JJC11.6)

For research use only
Capacity: for 30 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-099-698 $95.00

CD158i (KIR2DS4)-PE, human (clone: JJC11.6)

For research use only
Capacity: for 100 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-092-680 $265.00

CD158i (KIR2DS4)-APC, human (clone: JJC11.6)

For research use only
Capacity: for 30 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-099-709 $120.00

CD158i (KIR2DS4)-APC, human (clone: JJC11.6)

For research use only
Capacity: for 100 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-092-681 $280.00

CD158i (KIR2DS4)-PE-Vio770, human (clone: JJC11.6)

For research use only
Capacity: for 30 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-099-963 $140.00

CD158i (KIR2DS4)-PE-Vio770, human (clone: JJC11.6)

For research use only
Capacity: for 100 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-099-962 $290.00

CD158i (KIR2DS4)-Biotin, human (clone: JJC11.6)

For research use only
Capacity: for 100 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-092-898 $235.00

CD158i (KIR2DS4) pure, human (clone: JJC11.6)

For research use only
Content: 100 µg in 1 mL
 
Data sheet
130-092-679 $135.00

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Miltenyi Biotec Inc.
Phone: +1 800 FOR MACS
Fax:+1 877 591 1060
macs@miltenyibiotec.com
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