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CD158e/k antibodies, human (clone: 5.133)

Overview

Clone 5.133 reacts with CD158e (KIR3DL1) and CD158k (KIR3DL2), members of the killer immunoglobulin-like receptor (KIR) family which recognizes subsets of HLA alleles. The family of killer immunoglobulin-like receptors (KIR) contributes to the regulation of NK cell-mediated cytotoxicity. Expression is found mainly on CD56dimCD16+ natural killer (NK) cells but also on a subset of CD8+ T cells. They are monomeric receptors possessing high allelic polymorphism with either 2 or 3 Ig-like extracellular domains. According to the length of their cytoplasmic tail, KIRs can be subdivided in inhibitory KIRs and activating KIRs.

Technical data


Antigen CD158e/k (KIR3DL1/DL2)
Clone 5.133
Isotype mouse IgG1
Isotype control Mouse IgG1 – isotype control antibodies
Alternative names of antigen KIR3DL1, KIR3DL2
Entrez Gene ID 3811
Molecular mass of antigen [kDa] 46-48
Cross-reactivity
Distribution of antigen NK cells, T cells
Endotoxin level
Product format Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
Fixation Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
Storage Store protected from light at 2–8 °C. Do not freeze.
Available conjugates PE, PE-Vio770, Biotin, pure

Gallery

Figure 1

Human peripheral blood mononuclear cells (PBMCs) were stained with CD158e/k (KIR3DL1/DL2) antibodies as well as with CD56 antibodies and analyzed by flow cytometry using the MACSQuant® Analyzer. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandems.

Related Items

Product Order no. Price

CD158e/k (KIR3DL1/DL2)-PE, human (clone: 5.133)

For research use only
Capacity: for 30 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-099-121 $95.00

CD158e/k (KIR3DL1/DL2)-PE, human (clone: 5.133)

For research use only
Capacity: for 100 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-095-205 $270.00

CD158e/k (KIR3DL1/DL2)-PE-Vio770, human (clone: 5.133)

For research use only
Capacity: for 30 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-099-958 $140.00

CD158e/k (KIR3DL1/DL2)-PE-Vio770, human (clone: 5.133)

For research use only
Capacity: for 100 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-099-941 $290.00

CD158e/k (KIR3DL1/DL2)-Biotin, human (clone: 5.133)

For research use only
Capacity: for 100 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-095-201 $235.00

CD158e/k (KIR3DL1/DL2) pure, human (clone: 5.133)

For research use only
Content: 100 µg in 1 mL
 
Data sheet
130-095-199 $135.00

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Miltenyi Biotec Inc.
Phone: +1 800 FOR MACS
Fax:+1 877 591 1060
macs@miltenyibiotec.com
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