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CD158b (KIR2DL2/DL3) antibodies, human (clone: DX27)

Overview

Clone DX27 reacts with CD158b1 (KIR2DL2), CD158b2 (KIR2DL3), and CD158j (KIR2DS2), members of the killer immunoglobulin-like receptor (KIR) family expressed on natural killer (NK) cells and subsets of T cells.
KIRs contribute to the regulation of NK cell–mediated cytotoxicity and possess high allelic polymorphism with either 2 or 3 Ig-like extracellular domains. According to the length of their cytoplasmic tail, KIRs can be further subdivided in inhibitory KIRs (KIR2DL or KIR3DL) and activating KIRs (KIR2DS or KIR3DS). CD158b provides an inhibitory signal on NK cell lytic activity upon interaction with HLA C (e.g. alleles HLA-Cw1, HLA-Cw3, HLA-Cw7) in an antigen-independent manner.

Technical data


Antigen CD158b (KIR2DL2/DL3)
Clone DX27
Isotype mouse IgG2aκ
Isotype control Mouse IgG2a – isotype control antibodies
Alternative names of antigen KIR2DL2, KIR2DL3
Entrez Gene ID 3803
Molecular mass of antigen [kDa] 35-36
Cross-reactivity
Distribution of antigen NK cells, T cells
Endotoxin level
Product format Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
Fixation Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
Storage Store protected from light at 2–8 °C. Do not freeze.
Available conjugates PE, APC, PerCP, PE-Vio770, pure

Gallery

Figure 1

Human peripheral blood mononuclear cells (PBMCs) were stained with CD158b (KIR2DL2/DL3) antibodies as well as with CD56 antibodies and analyzed by flow cytometry using the MACSQuant® Analyzer. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandems.

Related Items

Library

Selected references

  1. Farag, S. S. et al. (2002) Natural killer cell receptors: new biology and insights into the graft-versus-leukaemia effect. Blood 100(6): 1935–1947.
  2. Djeu, J. et al. (2002) A view to a kill: signals triggering cytotoxicity. Clin. Cancer Res. 8: 636–640.
  3. Hsu, K. et al. (2002) The killer cell immunoglobin-like (KIR) genomic region: gene-order, haplotypes and allelic polymorphism. Immunol. Rev. 190: 40–52.
  4. Selvakumar, A. et al. (1997) Polymorphism and domain variability of human killer cell inhibitory receptors. Immunol. Rev. 155: 183–196.

Product Order no. Price

CD158b (KIR2DL2/DL3)-PE, human (clone: DX27)

For research use only
Capacity: for 30 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-099-397 price on request here

CD158b (KIR2DL2/DL3)-PE, human (clone: DX27)

For research use only
Capacity: for 100 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-092-618 price on request here

CD158b (KIR2DL2/DL3)-APC, human (clone: DX27)

For research use only
Capacity: for 30 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-099-378 price on request here

CD158b (KIR2DL2/DL3)-APC, human (clone: DX27)

For research use only
Capacity: for 100 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-092-617 price on request here

CD158b (KIR2DL2/DL3)-PerCP, human (clone: DX27)

For research use only
Capacity: for 30 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-099-700 price on request here

CD158b (KIR2DL2/DL3)-PerCP, human (clone: DX27)

For research use only
Capacity: for 100 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-095-285 price on request here

CD158b (KIR2DL2/DL3)-PE-Vio770, human (clone: DX27)

For research use only
Capacity: for 30 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-099-939 price on request here

CD158b (KIR2DL2/DL3)-PE-Vio770, human (clone: DX27)

For research use only
Capacity: for 100 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-099-892 price on request here

CD158b (KIR2DL2/DL3) pure, human (clone: DX27)

For research use only
Content: 100 µg in 1 mL
 
Data sheet
130-092-615 price on request here

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