Clone 11PB6, also referred to as EB6, recognizes CD158a (KIR2DL1) and CD158h (KIR2DS1), two members of the killer immunoglobulin-like receptor (KIR) family expressed on CD56dimCD16+ natural killer (NK) cells and CD8+ T cells. Findings also showed reactivity of 11PB6 with KIR2DL3*005.
CD158a (KIR2DL1) is involved in the transduction of an inhibitory signal whereas CD158h (KIR2DS1) is an activating receptor. The ligands of CD158a (KIR2DL1) are HLA-Cw4 and related molecules.
Human peripheral blood mononuclear cells (PBMCs) were stained with CD158a/h (KIR2DL1/DS1) antibodies as well as with CD56 antibodies and analyzed by flow cytometry using the MACSQuant® Analyzer. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.