Products and Services - MACS Flow Cytometry - Antibodies - Primary antibodies - Anti-TCR Vα7.2 antibodies, human

Anti-TCR Vα7.2 antibodies, human (clone: REA179)

Overview

Clone REA179 recognizes the TCR chain Vα7.2, which together with Jα33 segment forms the invariant T cell receptor. Human Vα7.2-Jα33 invariant T cells are preferentially localized in the gut and are often termed as mucosal-associated T (MAIT) cells. MAIT cells are restricted to MHC class I like molecules, such as MR1. Further, MAIT cells are suggested to be involved in regulation of immune response at the mucosal surfaces, which are the primary sites for pathogen entry.
Additional information: Clone REA179 displays negligible binding to Fc receptors.

Technical data


Antigen TCR Vα7.2
Clone REA179
Isotype recombinant human IgG1
Isotype control REA Control (S) antibodies
Alternative names of antigen TRA, IMD7, TCRA, TCRD, TRA, TRAC, TCRAV7S1, TRAV7
Entrez Gene ID 28686
Molecular mass of antigen [kDa]
Cross-reactivity
Distribution of antigen T cells
Endotoxin level
Product format Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
Fixation The antibody is suited for staining of formaldehyde-fixed cells.
Storage Store protected from light at 2–8 °C. Do not freeze.
Available conjugates FITC, PE, APC, VioBlue, PE-Vio770, APC-Vio770, PerCP-Vio700, Biotin

Gallery

Figure 1

Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-TCR Vα7.2 antibodies as well as with CD3 antibodies and analyzed by flow cytometry using the MACSQuant® Analyzer. The specificity of the conjugated antibodies was confirmed by blocking the binding to the ligand, using pure unconjugated antibodies (left images). The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.

Related Items

Library

Selected references

  1. Treiner, E. et al. (2003) Selection of evolutionarily conserved mucosal-associated invariant T cells by MR1. Nature 422(6928): 164–169.
  2. Qualai, J. et al. (2016) Expression of CD11c Is Associated with Unconventional Activated T Cell Subsets with High Migratory Potential. PLoS One 11.
  3. Gold, M. C. and Lewinsohn, D. M. (2013) Co-dependents: MR1-restricted MAIT cells and their antimicrobial function. Nat. Rev. Microbiol. 11(1): 14–19.

Product Order no. Price

Anti-TCR Vα7.2-FITC, human (clone: REA179)

For research use only
Capacity: for 30 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-100-190 $81.00

Anti-TCR Vα7.2-FITC, human (clone: REA179)

For research use only
Capacity: for 100 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-100-189 $228.00

Anti-TCR Vα7.2-PE, human (clone: REA179)

For research use only
Capacity: for 30 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-100-192 $96.00

Anti-TCR Vα7.2-PE, human (clone: REA179)

For research use only
Capacity: for 100 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-100-191 $268.00

Anti-TCR Vα7.2-APC, human (clone: REA179)

For research use only
Capacity: for 30 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-100-178 $122.00

Anti-TCR Vα7.2-APC, human (clone: REA179)

For research use only
Capacity: for 100 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-100-177 $284.00

Anti-TCR Vα7.2-VioBlue, human (clone: REA179)

For research use only
Capacity: for 30 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-100-211 $122.00

Anti-TCR Vα7.2-VioBlue, human (clone: REA179)

For research use only
Capacity: for 100 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-100-209 $294.00

Anti-TCR Vα7.2-PE-Vio770, human (clone: REA179)

For research use only
Capacity: for 30 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-100-207 $144.00

Anti-TCR Vα7.2-PE-Vio770, human (clone: REA179)

For research use only
Capacity: for 100 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-100-206 $296.00

Anti-TCR Vα7.2-APC-Vio770, human (clone: REA179)

For research use only
Capacity: for 30 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-100-180 $134.00

Anti-TCR Vα7.2-APC-Vio770, human (clone: REA179)

For research use only
Capacity: for 100 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-100-179 $305.00

Anti-TCR Vα7.2-PerCP-Vio700, human (clone: REA179)

For research use only
Capacity: for 30 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-100-204 $128.00

Anti-TCR Vα7.2-PerCP-Vio700, human (clone: REA179)

For research use only
Capacity: for 100 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-100-203 $305.00

Anti-TCR Vα7.2-Biotin, human (clone: REA179)

For research use only
Capacity: for 30 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-100-188 $86.00

Anti-TCR Vα7.2-Biotin, human (clone: REA179)

For research use only
Capacity: for 100 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-100-187 $228.00


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