Clone REA179 recognizes the TCR chain Vα7.2, which together with Jα33 segment forms the invariant T cell receptor. Human Vα7.2-Jα33 invariant T cells are preferentially localized in the gut and are often termed as mucosal-associated T (MAIT) cells. MAIT cells are restricted to MHC class I like molecules, such as MR1. Further, MAIT cells are suggested to be involved in regulation of immune response at the mucosal surfaces, which are the primary sites for pathogen entry.
Additional information: Clone REA179 displays negligible binding to Fc receptors.
Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-TCR Vα7.2 antibodies as well as with CD3 antibodies and analyzed by flow cytometry using the MACSQuant® Analyzer. The specificity of the conjugated antibodies was confirmed by blocking the binding to the ligand, using pure unconjugated antibodies (left images). The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.