Clone REA261 recognizes the killer cell lectin-like receptor subfamily G member 1 (KLRG1) antigen, a transmembrane protein, which is also known as C-type lectin domain family 15 member A (CLEC15A) or ITIM-containing receptor MAFA-L. KLRG1 is expressed by basophils, CD4 and CD8 T cells that exhibit a memory cell phenotype, and by a large proportion of peripheral blood NK cells. Expression of KLRG1 by virus-specific CD8+ T cells is induced by repetitive antigen stimulation and it differs in chronic versus resolved viral infections. T cells expressing KLRG1 exhibit a poor proliferation potential, while their ability to secrete gamma interferon is preserved. Thus, KLRG1 is a convenient marker for early T cell–differentiation.
Additional information: Clone REA261 displays negligible binding to Fc receptors.
Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-KLRG1 antibodies as well as with CD56 antibodies and analyzed by flow cytometry using the MACSQuant® Analyzer. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.