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Anti-KLRG1 antibodies, human (clone: REA261)

Overview

Clone REA261 recognizes the killer cell lectin-like receptor subfamily G member 1 (KLRG1) antigen, a transmembrane protein, which is also known as C-type lectin domain family 15 member A (CLEC15A) or ITIM-containing receptor MAFA-L. KLRG1 is expressed by basophils, CD4 and CD8 T cells that exhibit a memory cell phenotype, and by a large proportion of peripheral blood NK cells. Expression of KLRG1 by virus-specific CD8+ T cells is induced by repetitive antigen stimulation and it differs in chronic versus resolved viral infections. T cells expressing KLRG1 exhibit a poor proliferation potential, while their ability to secrete gamma interferon is preserved. Thus, KLRG1 is a convenient marker for early T cell–differentiation.
Additional information: Clone REA261 displays negligible binding to Fc receptors.

Technical data


Antigen KLRG1
Clone REA261
Isotype recombinant human IgG1
Isotype control REA Control (S) antibodies
Alternative names of antigen Killer cell lectin-like receptor subfamily G member 1, CLEC15A, MAFA, MAFA-2F1, MAFA-L, 2F1
Entrez Gene ID 10219
Molecular mass of antigen [kDa] 22
Cross-reactivity
Distribution of antigen basophils, T cells, NK cells
Endotoxin level
Product format Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
Fixation The antibody is suited for staining of formaldehyde-fixed cells.
Storage Store protected from light at 2–8 °C. Do not freeze.
Available conjugates FITC, PE, APC, PE-Vio615, PE-Vio770, APC-Vio770, Biotin

Gallery

Figure 1

Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-KLRG1 antibodies as well as with CD56 antibodies and analyzed by flow cytometry using the MACSQuant® Analyzer. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.

Related Items

Library

Selected references

  1. Voehringer, D. et al. (2002) Lack of proliferative capacity of human effector and memory T cells expressing killer cell lectinlike receptor G1 (KLRG1). Blood 100: 3698–3702.
  2. Thimme, R. et al. (2005) Increased expression of the NK cell receptor KLRG1 by virus-specific CD8 T cells during persistent antigen stimulation. J. Virol. 79: 12112–12116.
  3. Butcher, S. et al. (1998) MAFA-L, an ITIM-containing receptor encoded by the human NK cell gene complex and expressed by basophils and NK cells. Eur. J. Immunol. 28(11): 3755–3762.

Product Order no. Price

Anti-KLRG1-FITC, human (clone: REA261)

For research use only
Capacity: for 30 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-103-705 $80.00

Anti-KLRG1-FITC, human (clone: REA261)

For research use only
Capacity: for 100 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-103-640 $225.00

Anti-KLRG1-PE, human (clone: REA261)

For research use only
Capacity: for 30 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-103-703 $100.00

Anti-KLRG1-PE, human (clone: REA261)

For research use only
Capacity: for 100 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-103-638 $265.00

Anti-KLRG1-APC, human (clone: REA261)

For research use only
Capacity: for 30 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-103-704 $120.00

Anti-KLRG1-APC, human (clone: REA261)

For research use only
Capacity: for 100 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-103-639 $280.00

Anti-KLRG1-PE-Vio615, human (clone: REA261)

For research use only
Capacity: for 30 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-108-395 $135.00

Anti-KLRG1-PE-Vio615, human (clone: REA261)

For research use only
Capacity: for 100 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-108-366 $290.00

Anti-KLRG1-PE-Vio770, human (clone: REA261)

For research use only
Capacity: for 30 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-103-706 $140.00

Anti-KLRG1-PE-Vio770, human (clone: REA261)

For research use only
Capacity: for 100 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-103-641 $290.00

Anti-KLRG1-APC-Vio770, human (clone: REA261)

For research use only
Capacity: for 30 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-103-707 $130.00

Anti-KLRG1-APC-Vio770, human (clone: REA261)

For research use only
Capacity: for 100 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-103-642 $295.00

Anti-KLRG1-Biotin, human (clone: REA261)

For research use only
Capacity: for 30 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-103-762 $85.00

Anti-KLRG1-Biotin, human (clone: REA261)

For research use only
Capacity: for 100 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-103-751 $235.00


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