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Anti-Ki-67 antibodies, human and mouse (clone: REA183)

Overview

Clone REA183 recognizes Ki-67 antigen, a nuclear and nucleolar protein, which is strictly associated with cell proliferation. Two Ki-67 isoforms with molecular weight, 395 and 345 kDa are known. Both isoforms contain 16 “Ki-67 repeat“ sequences, each of which includes a conserved 66 bp “Ki-67 motif“. Expression of Ki-67 protein is present during all active phases of the cell cycle (G(1), S, G(2), and mitosis), but is absent from resting cells (G(0)). Due to strict association of Ki-67 with proliferating cells, Ki-67 is often used as an indicator of the “growth fraction“ of a given cell population. The N-terminal portion of Ki-67 contains a forkhead associated (FHA)1 domain, which is involved in interaction with proteins such as Hklp2 and NIFK.
Additional information: Clone REA183 displays negligible binding to Fc receptors.

Technical data


Antigen Ki-67
Clone REA183
Isotype recombinant human IgG1
Isotype control REA Control (I) antibodies
Alternative names of antigen MKI67, KIA, MIB-, MIB-1, PPP1R105, Ki67
Entrez Gene ID 4288
Molecular mass of antigen [kDa] 359
Cross-reactivity
Distribution of antigen
Endotoxin level
Product format Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
Fixation
Storage Store protected from light at 2–8 °C. Do not freeze.
Available conjugates FITC, PE, APC, PE-Vio615, PE-Vio770, PerCP-Vio700, Vio515, Vio667

Gallery

Figure 1

Jurkat cells were fixed, permeabilized, stained with Anti-Ki-67 antibodies, and analyzed by flow cytometry using the MACSQuant® Analyzer. The specificity of the conjugated antibodies was confirmed by blocking the binding to the ligand, using pure unconjugated antibodies (left peak). Cell debris and dead cells were excluded from the analysis based on scatter signals.

Figure 2

Figure 2
The 8 µm cryosection of the human tonsil was fixed with 100% ice-cold acetone for three minutes. The cryosection was stained with the CD19 antibodies, clone LT19 (red), Anti-Ki-67, clone REA183 antibodies (green), and CD3 antibodies, clone BW264/56 (blue).
The 8 µm cryosection of the human tonsil was fixed with 100% ice-cold acetone for three minutes. The cryosection was stained with the CD19 antibodies, clone LT19 (red), Anti-Ki-67, clone REA183 antibodies (green), and CD3 antibodies, clone BW264/56 (blue).

Related Items

Library

Selected references

  1. Verheijen, R. et al. (1989) Ki-67 detects a nuclear matrix-associated proliferation-related antigen. II. Localization in mitotic cells and association with chromosomes. J. Cell. Sci. 92: 531–540.
  2. Takagi, M. et al. (2001) A novel nucleolar protein, NIFK, interacts with the forkhead associated domain of Ki-67 antigen in mitosis. J. Biol. Chem. 276: 25386–25391.
  3. Jackson-Jones et al. (2016) Fat-associated lymphoid clusters control local IgM secretion during pleural infection and lung inflammation. Nat Commun 7: 12651.
  4. Schlöder, J. et al. (2017) Dimethyl Fumarate Therapy Significantly Improves the Responsiveness of T Cells in Multiple Sclerosis Patients for Immunoregulation by Regulatory T Cells. Int J Mol Sci 18.

Product Order no. Price

Anti-Ki-67-FITC, human and mouse (clone: REA183)

For research use only
Capacity: for 30 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-100-340 $80.00

Anti-Ki-67-FITC, human and mouse (clone: REA183)

For research use only
Capacity: for 100 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-100-339 $225.00

Anti-Ki-67-PE, human and mouse (clone: REA183)

For research use only
Capacity: for 30 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-100-290 $100.00

Anti-Ki-67-PE, human and mouse (clone: REA183)

For research use only
Capacity: for 100 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-100-289 $265.00

Anti-Ki-67-APC, human and mouse (clone: REA183)

For research use only
Capacity: for 30 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-100-332 $120.00

Anti-Ki-67-APC, human and mouse (clone: REA183)

For research use only
Capacity: for 100 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-100-330 $280.00

Anti-Ki-67-PE-Vio615, human and mouse (clone: REA183)

For research use only
Capacity: for 30 tests
 
Data sheet
130-107-203 $135.00

Anti-Ki-67-PE-Vio615, human and mouse (clone: REA183)

For research use only
Capacity: for 100 tests
 
Data sheet
130-107-150 $290.00

Anti-Ki-67-PE-Vio770, human and mouse (clone: REA183)

For research use only
Capacity: for 30 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-100-291 $140.00

Anti-Ki-67-PE-Vio770, human and mouse (clone: REA183)

For research use only
Capacity: for 100 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-100-288 $300.00

Anti-Ki-67-PerCP-Vio700, human and mouse (clone: REA183)

For research use only
Capacity: for 30 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-100-293 $130.00

Anti-Ki-67-PerCP-Vio700, human and mouse (clone: REA183)

For research use only
Capacity: for 100 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-100-292 $295.00

Anti-Ki-67-Vio515, human and mouse (clone: REA183)

For research use only
Capacity: for 30 tests
 
Data sheet
130-108-856 $130.00

Anti-Ki-67-Vio515, human and mouse (clone: REA183)

For research use only
Capacity: for 100 tests
 
Data sheet
130-108-830 $325.00

Anti-Ki-67-Vio667, human and mouse (clone: REA183)

For research use only
Capacity: for 30 tests
 
Data sheet
130-111-929 $165.00

Anti-Ki-67-Vio667, human and mouse (clone: REA183)

For research use only
Capacity: for 100 tests
 
Data sheet
130-111-761 $335.00


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