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Anti-IL-3 antibodies, mouse (clone: MP2-8F8)

Overview

IL-3 is a hematopoietic growth factor, which is produced mainly by activated T cells. IL-3 is also secreted by other cell types, including mast cells, eosinophils, and keratinocytes. The broad spectrum of biological activities of IL-3 includes the stimulation of the proliferation and differentiation of immature pluripotent hematopoietic stem cells and various lineage-committed progenitor cells, leading to the production of most of the major blood cell types. In addition, IL-3 also affects the functional activity of mature mast cells, basophils, eosinophils, and macrophages.

Technical data


Antigen IL-3
Clone MP2-8F8
Isotype rat IgG1κ
Isotype control Rat IgG1 – isotype control antibodies
Alternative names of antigen
Entrez Gene ID 16187
Molecular mass of antigen [kDa]
Cross-reactivity
Distribution of antigen
Endotoxin level
Product format Antibodies are supplied in buffer containing stabilizer and 0.05% sodium azide.
Fixation The antibody is suited for staining of formaldehyde-fixed cells.
Storage Store protected from light at 2–8 °C. Do not freeze.
Available conjugates PE, APC

Gallery

Figure 1

Naive CD4+ CD62L+ T cells were isolated using the CD4+CD62L+ T Cell Isolation Kit II from mouse spleen cells and stimulated with CD3ε pure – functional grade and CD28 pure – functional grade antibodies in the presence of recombinant Mouse IL-2 and Mouse IL-4 and Anti-IFN-γ pure – functional grade antibody for four days. Then cells were harvested, washed, and incubated with or without PMA/Ionomycin for six hours. After two hours, brefeldin A was added. The cells were harvested, fixed, permeabilized, and intracellularly stained with Anti-IL-3 conjugated to PE (B) or APC (C), CD4-FITC and CD154-APC or CD154-PE. Cells were analyzed by flow cytometry using the MACSQuant® Analyzer. Gating was performed according to the CD4 expression and side scatter properties of the cells. Cell debris was excluded from the analysis in a Propidium iodide versus Anti-IL-3-PE dot plot (A).
A: Analysis gate 1
Analysis gate 2
B: Stimulated sample
Unstimulated control
C: Stimulated sample
Unstimulated control

Related Items

Product Order no. Price

Anti-IL-3-PE, mouse (clone: MP2-8F8)

For research use only
Content: 30 µg in 1 mL (1)

Quantity

(1)200 tests. One test corresponds to labeling of up to 106 cells in a final volume of 50 µL.
 
Data sheet
130-102-437 $50.00

Anti-IL-3-APC, mouse (clone: MP2-8F8)

For research use only
Content: 30 µg in 1 mL (1)

Quantity

(1)200 tests. One test corresponds to labeling of up to 106 cells in a final volume of 50 µL.
 
Data sheet
130-102-402 $75.00

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Miltenyi Biotec Inc.
Phone: +1 800 FOR MACS
Fax:+1 877 591 1060
macs@miltenyibiotec.com
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