The DO11.10 mouse model is a valuable tool for studies of T cell immigration, immunoregulation, development, activation, and function. The KJ1-26 monoclonal antibody reacts with the T cell receptor (TCR) expressed on lymphocytes of the DO11.10 transgenic mouse and the TCR of the BALB/c-derived DO11.10 and DO11.10.24 T cell hybridoma. The DO11.10 TCR is specific for the chicken ovalbumin (OVA) peptide (323-339) in the context of I-A[d] major histocompatibility (MHC) molecules. Transgenic DO11.10 T cells also recognize OVA peptide from jungle fowl and turkey in the presence of A20-1.11, the H-2d-bearing, antigen-presenting B cell lymphoma.
Spleen cells from DO11.10 transgenic mice were stained with Anti-DO11.10 TCR antibodies conjugated to (A) FITC (B) PE (C) APC (D) VioBlue as well as with CD4-PE or CD4-APC and analyzed by flow cytometry using the MACSQuant® Analyzer. Cells labeled with Anti-DO11.10 TCR-Biotin (E) were stained with Anti-Biotin-PE as well as CD4-APC. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.