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Anti-DO11.10 TCR antibodies, mouse (clone: KJ1-26)

Overview

The DO11.10 mouse model is a valuable tool for studies of T cell immigration, immunoregulation, development, activation, and function. The KJ1-26 monoclonal antibody reacts with the T cell receptor (TCR) expressed on lymphocytes of the DO11.10 transgenic mouse and the TCR of the BALB/c-derived DO11.10 and DO11.10.24 T cell hybridoma. The DO11.10 TCR is specific for the chicken ovalbumin (OVA) peptide (323-339) in the context of I-A[d] major histocompatibility (MHC) molecules. Transgenic DO11.10 T cells also recognize OVA peptide from jungle fowl and turkey in the presence of A20-1.11, the H-2d-bearing, antigen-presenting B cell lymphoma.

Technical data


Antigen DO11.10 TCR
Clone KJ1-26
Isotype mouse IgG2a
Isotype control Mouse IgG2a – isotype control antibodies
Alternative names of antigen
Entrez Gene ID 21473
Molecular mass of antigen [kDa]
Cross-reactivity
Distribution of antigen T cells
Endotoxin level
Product format Antibodies are supplied in buffer containing stabilizer and 0.05% sodium azide.
Fixation Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
Storage Store protected from light at 2–8 °C. Do not freeze.
Available conjugates FITC, PE, APC, VioBlue, Biotin

Gallery

Figure 1

Spleen cells from DO11.10 transgenic mice were stained with Anti-DO11.10 TCR antibodies conjugated to (A) FITC (B) PE (C) APC (D) VioBlue as well as with CD4-PE or CD4-APC and analyzed by flow cytometry using the MACSQuant® Analyzer. Cells labeled with Anti-DO11.10 TCR-Biotin (E) were stained with Anti-Biotin-PE as well as CD4-APC. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
A:
B:
C:
D:
E:

Related Items

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Anti-DO11.10 TCR-FITC, mouse (clone: KJ1-26)

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Content: 30 µg in 1 mL (1)

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Anti-DO11.10 TCR-PE, mouse (clone: KJ1-26)

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Anti-DO11.10 TCR-APC, mouse (clone: KJ1-26)

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Anti-DO11.10 TCR-VioBlue, mouse (clone: KJ1-26)

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Anti-DO11.10 TCR-Biotin, mouse (clone: KJ1-26)

For research use only
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Miltenyi Biotec Inc.
Phone: +1 800 FOR MACS
Fax:+1 877 591 1060
macs@miltenyibiotec.com
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