Products and Services - MACS Flow Cytometry - Flow sorter

The revolution in cell sorting has begun

  • Gentle cell sorting in a closed cartridge system without sheath fluid
  • Engineered for operator safety with no aerosols or droplet formation
  • Intuitive handling suitable for any lab professional
The MACSQuant® Tyto™ is revolutionizing cell sorting. Our patented microchip-based technology opens up new possibilities in basic research and medical applications with high-speed multi-parameter flow sorting in the safety of a fully enclosed cartridge system, the MACSQuant® Tyto™ Cartridge.

MACSQuant® Tyto™ exclusive features:

Gentle to cells

Conventional droplet sorters use hydrodynamic focusing to singularize cells between sheath-fluids under high pressure. After laser detection cells get packed into little droplets when exiting the nozzle. Finally, a charge is applied to the droplets before they are sorted into collection tubes through an electromagnetic field.

It is well known that this process is extremely harsh to cells. Cells are exposed to high pressure, decompression when exiting the nozzle, application of charge and jet-in-air processing and collection, all of which may negatively impact cell viability and function.

The truly gentle sorting mechanism of the MACSQuant® Tyto™. Coming from the input chamber, cells enter the microchip through a microchannel where they get detected by the three lasers. Before entering the microchannel, potential cell aggregates are held back by a filter system guaranteeing a smooth sorting process. When a target cell (green) is identified, a magnetic pulse coming from the solenoid opens the microvalve, which then redirects the target cell into the positive collection chamber. In the default state, the valve is closed allowing non-selected cells (blue and red) to flow through into the negative collection chamber.

In contrast, the MACSQuant Tyto uses a truly gentle sorting technology. In the MACSQuant Tyto Cartridge, cells approach a micro-valve in a microfluidic channel. In contrast to conventional droplet sorters, cells are not exposed to hydrodynamic focusing so that high pressure can be avoided. After signal detection by three lasers, target cells are simply redirected into the positive or negative collection chamber by the micro-valve. Due to the absence of droplet formation, decompression and charge, this unique sorting mechanism enables the sorting of some of the most sensitive cell types such as granulocytes and stem cells without affecting cell viability and function. The gentle mode of sorting and the fact that both positive and negative cells are preserved within the Tyto Cartridge after each sort, easily allows for further sorting of either cell population on a new Tyto Cartridge.

Full safety

The MACSQuant Tyto Cartridge provides a fully closed system. Unlike conventional droplet sorters, the MACSQuant Tyto does not operate with a fluidics system. Instead, the instrument applies very mild air pressure (3 psi) on top of the sample loaded into the input chamber in order to drive cells onto the microchip for sorting.

Easy sample administration and collection processes are enabled by aseptic ports, which are incorporated into the Tyto Cartridge. In contrast to conventional sorting instruments, no biohazardous aerosols or droplets form during the sorting process with the MACSQuant Tyto. These unique features result in a minimized operator risk without the need for a biosafety cabinet.

Full sterility

Your samples are protected from environmental contamination in the closed cartridge system of the MACSQuant Tyto. The disposable Tyto Cartridge eliminates inter-sample spillover and the need of time-consuming washing and sterilization procedures. Therefore, it is assured that sorted cells are immediately available for further use in sensitive downstream applications such as cell culture, cell expansion, and medical applications. Importantly, positively- as well as negatively sorted cells remain in the Tyto Cartridge and can easily be retrieved after the sort, which offers a unique advantage over conventional sorting techniques. This may be of special interest when working with important samples or rare patient material.

Fast & easy

The MACSQuant Tyto is easily operated by lab professionals without the need for intensive training. Loading the Tyto Cartridge into the instrument proceeds in an intuitive “plug-and-play” fashion.

Sorting on the MACSQuant Tyto happens in four easy steps:

  1. The Tyto Cartridge with target cells is loaded into the machine
  2. A pre-defined sort profile is selected for the relevant target cells
  3. User presses ‘Play’ button and the machine automatically initiates the laser alignment routine
  4. Via image recognition, the precise laser interrogation position is determined and the sort process starts

MACSQuant Tyto is a truly walk-away flow-sorter that eliminates the need for specialized technical expertise during daily operation. Unlike most conventional droplet-sorters, the MACSQuant Tyto offers a fully automated laser alignment function. Due to the absence of droplets, droplet-stream positioning and re-adjustments are no longer needed. Since the entire sorting-process and cell-collection happens only on the Tyto Cartridge, there is no fluidic tubing present in the MACSQuant Tyto instrument eliminating maintenance work such as a fluidic-startup and cleaning procedure after the sorting process. These features in combination with the fact that the operator can leave the instrument unattended during the actual sorting process guarantee the most time and thus cost-effective workflows.

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Product Order no. Price

MACSQuant Tyto

Content: 1 unit
130-103-931 price on request here

Installation Service MACSQuant Tyto

160-002-055 price on request here

MACSQuant Tyto Cartridges

Content: 24 pieces
130-106-088 price on request here

MACSQuant Tyto Full Service

160-002-053 price on request here

MACSQuant Tyto One‑Time Planned Maintenance

160-002-052 price on request here

MACSQuant Tyto Planned Maintenance

160-002-051 price on request here

MACSQuant Tyto Running Buffer

Content: 6×100 mL
130-107-207 price on request here

MACSQuant Tyto Running Buffer

Content: 100 mL
130-107-206 price on request here
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Fax:+1 877 591 1060
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