Cell isolation directly from whole blood or bone marrow

  • No density gradient centrifugation, no erythrocyte lysis
  • Automation for standardization and ease of use
  • Excellent purities and recoveries from every sample
MACS Whole Blood MicroBeads allow magnetic cell sorting directly from whole blood or bone marrow. Leukocyte subsets are purified by using the autoMACS Pro Separator or the Whole Blood Column Kit with the MultiMACS Cell24 Separator Plus or manual separators. Pre-enrichment of white blood cells, e.g., by density gradient centrifugation, is not required. The purified cells are well-suited for further flow cytometric analysis, molecular biology studies, such as lineage-specific chimerism analysis, and functional studies.

Basic principle

StraightFrom™ Whole Blood MicroBeads were developed for rapid positive selection of cell subsets directly from whole blood or bone marrow. No sample preparation is required, including density gradient centrifugation or erythrocyte lysis. Anticoagulated samples of 0.25–15 mL are labeled with StraightFrom™ Whole Blood MicroBeads to be subsequently separated with the autoMACS Pro Separator or using the Whole Blood Column Kit.

Performing cell separations has never been easier with the autoMACS Pro Separator. This innovative instrument eliminates the need for manual sample handling steps, saving time while providing reliability and reproducibility in cell sorting. The autoMACS Pro Separator has been specifically designed for automated handling of multiple samples. State-of-the-art sensor technology allows safe operation and monitoring of the instrument’s status.

The Whole Blood Column Kit is suited for the separation of leukocyte subsets directly from anticoagulated human whole blood or bone marrow. The kit contains Whole Blood Columns and Whole Blood Column Elution Buffer for convenient positive selection of cells labeled with StraightFrom™ Whole Blood MicroBeads.

Use up to 9 Whole Blood Columns on the MultiMACS Cell24 Separator Plus in combination with the Single-Column Adapter to achieve the most convenient cell isolation directly from multiple whole blood samples.

This allows the isolation of pure and viable cells from up to 10 mL whole blood per sample. The Single-Column Adapter is part of the MultiMACS Cell24 Separator Plus.

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Miltenyi Biotec Inc.
Phone: +1 800 FOR MACS
Fax:+1 877 591 1060
macs@miltenyibiotec.com
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