Products and Services - MACS Cell Separation - MACS Technology - REAlease MicroBeads

REAlease™ MicroBeads for reversible cell labeling

  • Bead-free cells suited for second positive selection
  • Label-free cells for any downstream application
  • Recombinant antibody fragments for reproducible results
REAlease™ Technology allows for magnetic cell isolation and subsequent removal of any beads and labels from the cells in just a few simple steps. The technology relies on recombinantly engineered antibody fragments instead of whole antibodies for cell surface labeling. Monomeric fragments have a low affinity for cell surface markers. However, when multimerized as part of the REAlease Complex, the fragments bind markers with high avidity. REAlease MicroBeads bound to the REAlease Complex facilitate cell isolation using MACS® Columns and Separators. Disruption of the complex by adding REAlease Release Reagent after cell isolation leads to monomerization and spontaneous dissociation of the antibody fragments from the cell surface, resulting in label-free cells.

New opportunities in cell separation
REAlease Technology is an indirect cell labeling method for positive selection of target cells. The REAlease Biotin Complex binds to the target cells. Labeling of the REAlease Biotin Complex with Anti-Biotin MicroBeads allows for magnetic isolation of these cells. Following cell separation, both MicroBeads and REAlease Biotin Complex are gently removed, leaving the cells bead-and label-free. Enriched cells are then suitable for magnetic re-labeling and any application where label-free cells are essential.
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Product Order no. Price

REAlease CD3 MicroBead Kit, human

Capacity: for 1×109 total cells
 
130-117-038 price on request here

REAlease CD4 MicroBead Kit, human

Capacity: for 1×109 total cells
 
130-117-037 price on request here

REAlease CD8 MicroBead Kit, human

Capacity: for 1×109 total cells
 
130-117-036 price on request here

REAlease CD19 MicroBead Kit, human

Capacity: for 1×109 total cells
 
130-117-034 price on request here

REAlease CD56 MicroBead Kit, human

Capacity: for 1×109 total cells
 
130-117-033 price on request here
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