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NK Cell Isolation Kit, mouse

  • Effective: excellent NK cell purity
  • Trouble-free: isolated NK cells are fully functional
  • Unrivaled: superior T cell depletion

Overview

The NK Cell Isolation Kit was developed for the isolation of untouched NK cells from single-cell suspensions of murine spleen. Non-NK cells, i.e. T cells, dendritic cells, B cells, granulocytes, macrophages, and erythroid cells are magnetically labeled by using a cocktail of biotin-conjugated antibodies and Anti-Biotin MicroBeads. Isolation of highly pure unlabeled NK cells is achieved by depletion of non-target cells. The kit has been optimized to give outstanding purities in C57BL/6J mice.

Details

Downstream applications

NK cells isolated using the NK Cell Isolation Kit can be used for in vitro and in vivo applications, e.g., functional studies or adoptive transfer experiments.

Columns

MS, LS, or autoMACS® Columns.

Gallery

Figure 1

NK cells were isolated from a BALB/c (A) or C57BL/6 (B) mouse spleen cell suspension by using the NK Cell Isolation Kit, an LS Column, and a MidiMACS™ Separator. The cells were fluorescently stained with CD3-FITC, CD49b-APC, and Anti-NKp46-PE (A), or CD3-FITC, CD49b-APC, and Anti-NK1.1-PE (B) and analyzed by flow cytometry using the MACSQuant® Analyzer 10. Cell debris and dead cells were excluded from the analysis based on scatter signals, CD45-VioGreen™, and 4’,6-diamidino-2-phenylindole(DAPI) fluorescence.

A) BALB/c mouse spleen cell suspension

Before separation
Isolated NK cells

B) C57BL/6 mouse spleen cell suspension

Before separation
Isolated NK cells

Related Items

Product Order no. Price

NK Cell Isolation Kit, mouse

Capacity: for 1×109 total cells
 
Data sheet
130-115-818 $655.00

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Miltenyi Biotec Inc.
Phone: +1 800 FOR MACS
Fax:+1 877 591 1060
macs@miltenyibiotec.com
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