Products and Services - MACS Cell Separation - Cell separation reagents - NK cells - CD56 MicroBeads, human

CD56 MicroBeads, human

  • Efficient: high yield of isolated CD56+ cells
  • Gentle: excellent viability rates
  • Time-saving: purified cells are immediately ready for any downstream application

Overview

CD56 MicroBeads were developed for the positive selection or depletion of natural killer (NK) cells from human PBMCs or single-cell suspensions from tissues. The CD56 antigen is expressed by most NK cells and a minor T cell subset (CD3+CD56+ NKT cells). Upon activation of NK cells, the surface expression of CD56 is increased.

Details

Downstream applications

CD56+ cells are used in studies on NK cell development, proliferation, or cytotoxic activity as well as in analysis of signal transduction and related molecular processes1–3.
CD56 may also be used for the enrichment of embryonic stem cell–derived cardiomyocytes cells after in vitro differentiation4 as well as the direct selection stem and progenitor cells from muscle tissue5,6.

Columns

For positive selection: MS, LS, XS, or autoMACS® Columns. For depletion: LD, CS, D, or autoMACS Columns.

Gallery

Figure 1

CD56+ cells were isolated from human peripheral blood mononuclear cells (PBMCs) using CD56 MicroBeads, an LS Column, and a MidiMACS™ Separator. Cells were fluorescently stained with CD56-PE and CD3-APC and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
PBMCs before separation
Isolated CD56+ cells

Related Items

Library

Selected references

  1. Fletcher, J. M. et al. (1998) Natural killer cell lysis of cytomegalovirus (CMV)-infected cells correlates with virally induced changes in cell surface lymphocyte function-associated antigen-3 (LFA-3) expression and not with the CMV-induced down-regulation of cell surface class I HLA. J. Immunol. 161: 2365–2374.
  2. Doherty, D. G. et al. (1999) The human liver contains multiple populations of NK cells, T cells, and CD3+CD56+ natural T cells with distinct cytotoxic activities and Tʜ1, Tʜ2, and Tʜ0 cytokine secretion patterns. J. Immunol. 163: 2314–2321.
  3. Kumaki, S. et al. (2001) Identification of anti-herpes simplex virus antibody-producing B cells in a patient with an atypical RAG1 immunodeficiency. Blood 98: 1464–1468.
  4. Xu, S. et al. (2006) Cardiac bodies: a novel culture method for enrichment of cardiomyocytes derived from human embryonic stem cells. Stem Cells Dev. 15: 631–639.
  5. Sinanan, A. et al. (2004) Human adult craniofacial muscle-derived cells: neural-cell adhesion-molecule (NCAM; CD56)-expressing cells appear to contain multipotential stem cells. Biotechnol. Appl. Biochem. 40: 25–34.
  6. De Luna, N. et al. (2006) Absence of dysferlin alters myogenin expression and delays human muscle differentiation in vitro. J. Biol. Chem. 281: 17092–17098.

Product Order no. Price

CD56 MicroBeads, human

Capacity: for 1×109 total cells
 
Data sheet
130-050-401 $640.00

CD56 MicroBeads, human – lyophilized

Capacity: for 1×109 total cells
 
Data sheet
130-097-042 $640.00

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Miltenyi Biotec Inc.
Phone: +1 800 FOR MACS
Fax:+1 877 591 1060
macs@miltenyibiotec.com
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