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CD3+CD56+ NKT Cell Isolation Kit, human

Overview

The CD3+CD56+ NKT Cell Isolation Kit was developed for the sequential separation of CD3+CD56+ natural killer (NK) T cells from human PBMCs and other single-cell suspensions.

Details

Background information

NKT cells represent a subpopulation of T cells that possesses properties of NK cells. NKT cells can be stimulated through contact with antigen or by cytokines such as IL-12 to release large amounts of cytokines and to exert cytotoxic effects. NKT cells are a crucial part of the innate immune system and are involved in the development of autoimmune diseases, in tumor immunology as well as in immunity against viruses, bacterial, fungal, and parasitic pathogens.

Detailed separation procedure

In a first step, NK cells and monocytes are magnetically labeled by using a cocktail of biotin-conjugated antibodies and Anti-Biotin MicroBeads. The labeled cells are subsequently depleted by separation over a MACS® Column. In the second step, CD3+CD56+ NKT cells are isolated by positive selection from the pre-enriched NKT cell fraction using CD56 MicroBeads.

Downstream applications

CD3+CD56+ NKT cells can be used for a variety of studies such as analysis of cytokine secretion, gene expression analysis of cell subsets, analysis of the functional role of cell surface receptors, cytotoxic and cytolytic activity.

Columns

For the first magnetic separation (depletion): LD or autoMACS® Columns. For the second magnetic separation (positive selection): MS or autoMACS Columns.

Gallery

Figure 1

CD3+CD56+ NKT cells were isolated from human PBMCs by using the CD3+CD56+ NKT Cell Isolation Kit, an LD Column and an MS Column, a MidiMACS™ Separator and a MiniMACS™ Separator. The cells were fluorescently stained with CD3-FITC and CD56-APC.
A: Unseparated fraction
B: After depletion
C: Enriched CD3+CD56+ NKT cells

Related Items

Product Order no. Price

CD3+CD56+ NKT Cell Isolation Kit, human

Capacity: for 2×109 total cells
 
Data sheet
130-093-064 $900.00

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Miltenyi Biotec Inc.
Phone: +1 800 FOR MACS
Fax:+1 877 591 1060
macs@miltenyibiotec.com
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