The Anti-NKp46 MicroBead Kit is an indirect magnetic labeling system for the enrichment of mouse NK cells. NKp46+ cells are labeled with Anti-NKp46-Biotin and Anti-Biotin MicroBeads for subsequent magnetic separation.
Due to the nature of the antibody, a limited degree of pre-activation might occur after cell separation with this product.
NKp46, also known as MAR-1 or CD335, is a type I transmembrane protein with two extracellular Ig-like domains. It is a member of the natural cytotoxicity receptor (NCR) family, which triggers cytotoxicity in NK cells. NKp46 is involved in target cell recognition and lysis and seems to be exclusively expressed on NK cells. Expression has been shown on BALB/c, SJL, CBA/CA, C57Bl/6, NOD, DBA/2, and B6.129 mice1.
MS or autoMACS® Columns.
NKp46+ NK cells were isolated from BALB/c mouse spleen using the Anti-NKp46 MicroBead Kit, two consecutive MS Columns, and a MiniMACS™ Separator. Cells were fluorescently stained with CD3ε-APC and Anti-NKp46-PE and analyzed by flow cytometry using the MACSQuant® Analyzer.