Products and Services - MACS Cell Separation - Cell separation reagents - Neural cells - CD11b (Microglia) MicroBeads, human and mouse

CD11b (Microglia) MicroBeads, human and mouse

Overview

CD11b (Microglia) MicroBeads have been developed for the positive isolation of primary mouse and human CD11b+ microglia. The purification protocol takes approx. one hour.

Details

Background information

Microglia, often called brain macrophages, are the resident immune effector cells in the central nervous system (CNS). In addition, activated microglia serve as the major antigen-presenting cells in the CNS.1 They are morphologically, immunophenotypically, and functionally related to cells of the monocyte/macrophage lineage.2
This detailed protocol using CD11b (Microglia) MicroBeads allows the fast isolation of primary microglia within 1h. The use of the MACS Neural Tissue Dissociation Kit-P prior to the purification leads to highest yield and viability.
For the isolation of microglia from myelin-containing tissue samples (human tissue or adult mouse or rat), the use of Myelin Removal Beads II is highly recommended.

Downstream applications

CD11b (Microglia) MicroBeads have been used to isolate CD11b+ cells from single-cell suspensions of brain tissue3-6.

Columns

MS, LS, XS, or autoMACS® Columns.

Gallery

Figure 1

Whole-brain tissue from P1 mice was first dissociated to single cells using the Neural Tissue Dissociation Kit (T) and then microglia were isolated using CD11b (Microglia) MicroBeads, an MS Column, and a MiniMACS™ Separator. Cells are fluorescently stained with CD11b-APC and CD45-FITC.
Before separation
Microglia CD11b+ fraction

Related Items

Library

Selected references

  1. Streit et al. (2004) Glia 1: 301–307.
  2. Guillemin et al. (2004) J. Leukoc. Biol. 75: 388–397.
  3. Boddaert et al. (2007) Am. J. Pathol. 170: 921–929.
  4. Li et al. (2006) J. Immunol. 177: 3644–3656.
  5. Ndhlovu et al. (2001) J. Immunol. 167: 2991–2999.
  6. Schulter et al. (2001) Brain Pathol. 11: 44–55.

Brochures and posters

Neuroscience research brochure [PDF, 4.9 MB, EN]

Scientific posters

Product Order no. Price

CD11b (Microglia) MicroBeads, human and mouse – small size

Capacity: for 5×107 total cells
 
Data sheet
130-093-636 $75.00

CD11b (Microglia) MicroBeads, human and mouse

Capacity: for 1×109 total human cells
 
Data sheet
130-093-634 $620.00

Customer login

Go

Forgot password?
Register for account

0 Shopping cart

Contact

Miltenyi Biotec Inc.
Phone: +1 800 FOR MACS
Fax:+1 530 745 2806
macs@miltenyibiotec.com
Contact overview

Product finder

Antibody panel builder

Toolbox

Play MACS Scientific Games

Play MACS Scientific Games

Change country

Please select your country to continue