CD11b (Microglia) MicroBeads have been developed for the positive isolation of primary mouse and human CD11b+ microglia. The purification protocol takes approx. one hour.
Microglia, often called brain macrophages, are the resident immune effector cells in the central nervous system (CNS). In addition, activated microglia serve as the major antigen-presenting cells in the CNS. They are morphologically, immunophenotypically, and functionally related to cells of the monocyte/macrophage lineage.
This detailed protocol using CD11b (Microglia) MicroBeads allows the fast isolation of primary microglia within 1h. The use of the MACS Neural Tissue Dissociation Kit-P prior to the purification leads to highest yield and viability.
For the isolation of microglia from myelin-containing tissue samples (human tissue or adult mouse or rat), the use of Myelin Removal Beads II is highly recommended.
CD11b (Microglia) MicroBeads have been used to isolate CD11b+ cells from single-cell suspensions of brain tissue3-6.
MS, LS, XS, or autoMACS® Columns.
Whole-brain tissue from P1 mice was first dissociated to single cells using the Neural Tissue Dissociation Kit (T) and then microglia were isolated using CD11b (Microglia) MicroBeads, an MS Column, and a MiniMACS™ Separator. Cells are fluorescently stained with CD11b-APC and CD45-FITC.
Microglia CD11b+ fraction