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CD11b (Microglia) MicroBeads, human and mouse

Overview

CD11b (Microglia) MicroBeads have been developed for the positive isolation of primary mouse and human CD11b+ microglia. The purification protocol takes approx. one hour.

Details

Background information

Microglia, often called brain macrophages, are the resident immune effector cells in the central nervous system (CNS). In addition, activated microglia serve as the major antigen-presenting cells in the CNS. They are morphologically, immunophenotypically, and functionally related to cells of the monocyte/macrophage lineage.
This detailed protocol using CD11b (Microglia) MicroBeads allows the fast isolation of primary microglia within 1 hour. The use of the MACS Neural Tissue Dissociation Kit (P) prior to the purification leads to highest yield and viability.
For the isolation of microglia from myelin-containing tissue samples (human tissue or adult mouse or rat), the use of Myelin Removal Beads II is highly recommended.

Downstream applications

CD11b (Microglia) MicroBeads have been used to isolate CD11b+ cells from single-cell suspensions of brain tissue3-6.

Columns

MS, LS, XS, or autoMACS® Columns.

Gallery

Figure 1

Whole-brain tissue from P1 mice was first dissociated to single cells using the Neural Tissue Dissociation Kit (T) and then microglia were isolated using CD11b (Microglia) MicroBeads, an MS Column, and a MiniMACS™ Separator. Cells are fluorescently stained with CD11b-APC and CD45-FITC.
Before separation
Microglia CD11b+ fraction

Miltenyi TV

Video 1

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How to isolate pure viable microglia

How to isolate pure viable microglia

Watch this hands-on protocol showing you how to isolate microglial cells from adult mouse brain. The Miltenyi Biotec protocol is faster, standardized, reproducible, and easy to scale up.

Related Items

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Selected references

  1. Justin, A. et al. (2012) The Ifng gene is essential for Vdr gene expression and vitamin D3-mediated reduction of the pathogenic T cell burden in the central nervous system in experimental autoimmune encephalomyelitis, a multiple sclerosis model. J. Immunol. 189: 3188–3197.
  2. Lauren, L. et al. (2011) Microglia and memory: modulation by early-life infection. J. Neurosci. 31: 15511–15521.
  3. Kremer, M. et al. (2010) Reduced immunoproteasome formation and accumulation of immunoproteasomal precursors in the brains of lymphocytic choriomeningitis virus-Infected mice. J. Immunol. 185: 5549–5560.
  4. Lee et al. (2008) J. Neurosci. 28: 8517–8528.
  5. Su, W. et al. (2014) The p53 transcription factor modulates microglia behavior through microRNA-dependent regulation of c-maf. J. Immunol. 192: 358–366.
  6. Zhang, X. et al. (2014) Peripheral role of cathepsin S in Tʜ1 cell-dependent transition of nerve injury-induced acute pain to a chronic pain state. J. Neurosci. 34: 3013–3022.
  7. Woodling, N. S. et al. (2014) Suppression of alzheimer-associated inflammation by microglial prostaglandin-E2 EP4 receptor signaling. J. Neurosci. 34: 5882–5894.
  8. Kleinberger, G. et al. (2014) TREM2 mutations implicated in neurodegeneration impair cell surface transport and phagocytosis. Sci Transl Med 6: 243ra86.
  9. Cho, S.-H. et al. (2015) SIRT1 deficiency in microglia contributes to cognitive decline in aging and neurodegeneration via epigenetic regulation of IL-1β. J. Neurosci. 35: 807–818.
  10. Zeiner, P. S. et al. (2014) MIF receptor CD74 is restricted to microglia/macrophages, associated with a M1-polarized immune milieu and prolonged patient survival in gliomas. Brain Pathol. 25: 491–504.
  11. Kiris, E. et al. (2015) Dysregulation of glutamine transporter SNAT1 in rett syndrome microglia: a mechanism for mitochondrial dysfunction and neurotoxicity. J. Neurosci. 35: 2516–2529.

Brochures and posters

Scientific posters

Product Order no. Price

CD11b (Microglia) MicroBeads, human and mouse – small size

Capacity: for 1×108 total cells
 
Data sheet
130-093-636 $75.00

CD11b (Microglia) MicroBeads, human and mouse

Capacity: for 1×109 total cells
 
Data sheet
130-093-634 $465.00

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Phone: +1 800 FOR MACS
Fax:+1 877 591 1060
macs@miltenyibiotec.com
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