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Anti-A2B5 MicroBeads, human, mouse, rat

Overview

Anti-A2B5 MicroBeads have been developed for positive selection of human, murine, and rat A2B5+ glial progenitor cells.

Details

Background information

A2B5 is predominantly expressed in embryonic and neonatal neural tissue. In adult mammalian brain A2B5 expression is restricted mainly to areas that retain neurogenic potential such as the subventricular zone (SVZ). Thus, A2B5 is considered to be a marker for immature glial-committed precursors that are permanently generated in the SVZ.
Ganglioside GT3 and its O-acetylated derivative are the principal A2B5-reactive gangliosides, and both antigens are down-regulated as the cells differentiate into mature oligodendrocytes.
Glial progenitors from embryonic to adult human, murine, and rat tissue have been immunomagnetically isolated according to the expression of A2B51–5.

Columns

MS, LS, or autoMACS® Columns.

Gallery

Figure 1

Isolation of A2B5+ cells from day 1 postnatal mouse whole brain tissue using the Neural Tissue Dissociation Kit (P), Anti-A2B5 MicroBeads, a MiniMACS™ Separator, and an MS Column.
Dissociated brain cells before separation
A2B5 cells
A2B5+ cells

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Selected references

  1. Windrem, M. S. et al. (2004) Fetal and adult human oligodendrocyte progenitor cell isolates myelinate the congenitally dysmyelinated brain. Nat. Med. 10: 93–97.
  2. Ruffini, F. et al. (2004) Distinctive properties of human adult brain-derived myelin progenitor cells. Am. J. Pathol. 165: 2167–2175.
  3. Larsen, P. H. et al. (2006) Myelin formation during development of the CNS is delayed in matrix metalloproteinase-9 and -12 null mice. J. Neurosci. 26: 2207–2214.
  4. Seidenfaden, R. et al. (2006) Glial conversion of SVZ-derived committed neuronal precursors after ectopic grafting into the adult brain. Mol. Cell. Neurosci. 32: 187–198.
  5. Larsen, P. H. and Yong, V. W. (2004) The expression of matrix metalloproteinase-12 by oligodendrocytes regulates their maturation and morphological differentiation. J. Neurosci. 24: 7597–7603.
  6. Liu, A. et al. (2007) The glial or neuronal fate choice of oligodendrocyte progenitors is modulated by their ability to acquire an epigenetic memory. J. Neurosci. 27: 7339–7343.
  7. Strathmann et al. (2007) Identification of two novel glial-restricted cell populations in the embryonic telencephalon arising from unique origins. BMC Dev. Biol. 7: 33.
  8. Larsen, P. H. et al. (2003) Matrix metalloproteinase-9 facilitates remyelination in part by processing the inhibitory NG2 proteoglycan. J. Neurosci. 23: 11127–11135.
  9. Dietrich, J. et al. (2002) Characterization of A2B5+ glial precursor cells from cryopreserved human fetal brain progenitor cells. Glia 40: 65–77.
  10. Dubois, C. et al. (1990) Monoclonal antibody A2B5, which detects cell surface antigens, binds to ganglioside GT3 (II3 (NeuAc)3LacCer) and to its 9-O-acetylated derivative. J. Biol. Chem. 265: 2797–2803.

Brochures and posters

Neuroscience research brochure [PDF, 1.9 MB, EN]

Product Order no. Price

Anti-A2B5 MicroBeads, human, mouse, rat – small size

Capacity: for 5×107 total cells
 
Data sheet
130-093-392 $75.00

Anti-A2B5 MicroBeads, human, mouse, rat

Capacity: for 5×108 total cells
 
Data sheet
130-093-388 $445.00

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Miltenyi Biotec Inc.
Phone: +1 800 FOR MACS
Fax:+1 877 591 1060
macs@miltenyibiotec.com
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