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Monocyte Isolation Kit II, human

  • Easy: one-step isolation of untouched monocytes
  • High-purity monocytes: perfectly suited for Mo-DC generation and other downstream applications

Overview

The Monocyte Isolation Kit II is an indirect magnetic labeling system for the isolation of untouched monocytes from human PBMCs. For a more efficient isolation of all monocytes including CD16+ monocytes, the Pan Monocyte Isolation Kit, human is recommended.

Details

Detailed separation procedure

Non-monocytes are indirectly magnetically labeled using a cocktail of biotin-conjugated antibodies as well as Anti-Biotin MicroBeads. Highly enriched unlabeled monocytes are obtained by depletion of the magnetically labeled cells.

Downstream applications

Monocytes isolated with the Monocyte Isolation Kit II are used in studies where direct labeling of surface molecules is undesired. Untouched monocytes are also used for further isolation of specialized cell subsets such as activated monocytes. The most eminent use of isolated monocytes is their ex vivo differentiation into dendritic cells.1,2

Columns

LS or autoMACS® Columns.

Gallery

Figure 1

Isolation of untouched monocytes from PBMCs using the Monocyte Isolation Kit II, an LS Column, and a MidiMACS™ Separator.
PBMCs before separation
Isolated untouched monocytes

Figure 2

Figure 2
Working scheme for the isolation of untouched monocytes using the Monocyte Isolation Kit II.
Working scheme for the isolation of untouched monocytes using the Monocyte Isolation Kit II.

Related Items

Library

Selected references

  1. Hu et al. (2004) J. Exp. Med. 199: 1065–1075.
  2. Hafsi et al. (2004) J. Immunol. 173: 1249–1257.

Customer reports and application notes

Product Order no. Price

Monocyte Isolation Kit II, human

Capacity: for 1×109 total cells
 
Data sheet
130-091-153 $700.00

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Miltenyi Biotec Inc.
Phone: +1 800 FOR MACS
Fax:+1 877 591 1060
macs@miltenyibiotec.com
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