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CD235a (Glycophorin A) MicroBeads, human

Overview

CD235a (Glycophorin A) MicroBeads can be used for the positive selection or depletion of human erythroid cells, e.g., from peripheral blood, buffy coat or cord blood.

Details

Background information

The CD235a antigen (glycophorin A), a single-pass transmembrane glycoprotein, is expressed on mature erythrocytes and erythroid precursor cells.

Downstream applications

Example applications include the enrichment of pluripotent stem cells from human bone marrow aspirate by a depletion strategy using CD235a (Glycophorin A) MicroBeads together with CD45 MicroBeads.1

Columns

For positive selection: MS, LS, XS, or autoMACS® Columns. For depletion: LD, CS, D, or autoMACS Columns.

Gallery

Figure 1

Separation of CD235a+ cells from cord blood mononuclear cells using CD235a (Glycophorin A) MicroBeads, an MS Column, and a MiniMACS™ Separator. Cells were stained with CD235a-FITC antibody and CD71-PE.
Cord blood mononuclear cells before separation
CD235a- cells
Isolated CD235a+ cells

Related Items

Library

Selected references

  1. Reyes, M. et al. (2001) Purification and ex vivo expansion of postnatal human marrow mesodermal progenitor cells. Blood 98: 2615–2625.

Product Order no. Price

CD235a (Glycophorin A) MicroBeads, human

Capacity: for 1×109 total cells
 
Data sheet
130-050-501 $665.00

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Miltenyi Biotec Inc.
Phone: +1 800 FOR MACS
Fax:+1 877 591 1060
macs@miltenyibiotec.com
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