CD235a (Glycophorin A) MicroBeads can be used for the positive selection or depletion of human erythroid cells, e.g., from peripheral blood, buffy coat or cord blood.
The CD235a antigen (glycophorin A), a single-pass transmembrane glycoprotein, is expressed on mature erythrocytes and erythroid precursor cells.
Example applications include the enrichment of pluripotent stem cells from human bone marrow aspirate by a depletion strategy using CD235a (Glycophorin A) MicroBeads together with CD45 MicroBeads.1
For positive selection: MS, LS, XS, or autoMACS® Columns. For depletion: LD, CS, D, or autoMACS Columns.
Separation of CD235a+ cells from cord blood mononuclear cells using CD235a (Glycophorin A) MicroBeads, an MS Column, and a MiniMACS™ Separator. Cells were stained with CD235a-FITC antibody and CD71-PE.
Cord blood mononuclear cells before separation
Isolated CD235a+ cells