The Tumor-Associated Fibroblast Isolation Kit, mouse has been designed for the isolation of tissue-derived, tumor-associated fibroblasts from dissociated mouse tumors.
The Tumor-Associated Fibroblast Isolation Kit, mouse enables the isolation of tissue-derived, tumor-associated fibroblasts from dissociated mouse tumors.
Tumor-associated fibroblasts have been shown to play an important role in tumor development, progression, and function. However, they may only make up about 0.5 – 5% of the total number of cells found in tumors. Applying a two-step strategy, tumor-associated fibroblasts are first pre-enriched by removing non-target cells, followed by isolation using the general fibroblast marker CD90.2. This allows reliable isolation from different syngeneic mouse tumor models.
For optimal results, the Tumor-Associated Fibroblast Isolation Kit, mouse should be used in combination with the Tumor Dissociation Kit, mouse (# 130-096-730) and the gentleMACS™ Dissociators.
MS, LD, or autoMACS® Columns.
Mouse tumor-associated fibroblasts were purified from a mouse B16-F10 tumor (melanoma) using the Tumor-Associated Fibroblast Isolation Kit, mouse. For pre-enrichment an LD Column and a QuadroMACS™ Separator were used, subsequent isolation was performed on a MS Column and a MiniMACS™ Separator. Cells were fluorescently stained with CD45-PE-Vio® 770 and CD90.2-APC, and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Pre-enriched tumor-associated fibroblasts
Isolated tumor-associated fibroblasts