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CD56 MultiSort Kit, human

Overview

The CD56 MultiSort Kit was developed for the sequential positive selection of CD56+ cells from human blood, bone marrow, cell cultures, or lymphoid tissue, allowing the isolation of distinct CD56+ cell subsets.

Details

Detailed separation procedure

CD56+ cells are isolated by positive selection and the magnetic particles are enzymatically released from the MultiSort MicroBeads. The selected CD56+ cells can then be magnetically labeled and sorted for a second antigen.

Downstream applications

The CD56 MultiSort Kit can be used to isolate specific NK or T cell subsets, e.g., CD8+CD56+, CD3+CD56+, or CD56+CD69+ cell populations.

Columns

MS, LS, XS, or autoMACS® Columns.

Gallery

Figure 1

CD8+CD56+ cells were separated from PBMCs using the CD56 MultiSort Kit and CD8 MicroBeads. Cells are stained with CD8-FITC and CD56-PE.
PBMCs before separation
CD56+ cells
CD8+CD56+

Figure 2

Figure 2
Step-by-step CD56 MultiSort separation procedure.
Step-by-step CD56 MultiSort separation procedure.

Related Items

Library

Selected references

  1. Santourlidis, S. et al. (2002) Crucial role of DNA methylation in determination of clonally distributed killer cell Ig-like receptor expression patterns in NK cells. J. Immunol. 169: 4253–4261.

Product Order no. Price

CD56 MultiSort Kit, human

Capacity: for 1×109 total cells
 
Data sheet
130-055-401 $720.00

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Miltenyi Biotec Inc.
Phone: +1 800 FOR MACS
Fax:+1 877 591 1060
macs@miltenyibiotec.com
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