CD56 MicroBeads were developed for the positive selection or depletion of natural killer (NK) cells from human PBMCs or single-cell suspensions from tissues. The CD56 antigen is expressed by most NK cells and a minor T cell subset (CD3+CD56+ NKT cells). Upon activation of NK cells, the surface expression of CD56 is increased.
CD56+ cells are used in studies on NK cell development, proliferation, or cytotoxic activity as well as in analysis of signal transduction and related molecular processes1–3.
CD56 may also be used for the enrichment of embryonic stem cell–derived cardiomyocytes cells after in vitro differentiation4 as well as the direct selection stem and progenitor cells from muscle tissue5,6.
For positive selection: MS, LS, XS, or autoMACS® Columns. For depletion: LD, CS, D, or autoMACS Columns.
CD56+ cells were isolated from human peripheral blood mononuclear cells (PBMCs) using CD56 MicroBeads, an LS Column, and a MidiMACS™ Separator. Cells were fluorescently stained with CD56-PE and CD3-APC and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
PBMCs before separation
Isolated CD56+ cells