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CD49b (DX5) MicroBeads, mouse

  • Efficient: outstanding yields of purified NK cells
  • Gentle: excellent cell viability
  • Broadening your research horizon: isolated cells are suited for adoptive transfer


Mouse CD49b (DX5) MicroBeads are used for the isolation of mouse NK cells from single-cell suspensions of lymphoid and non-lymphoid tissue, as well as from peripheral blood or body fluids.


Background information

CD49b is expressed on NK cells and a small population of T cells (CD4+CD3+ TCRα/β+). These cells can be depleted prior to NK cell enrichment by using CD90.2 MicroBeads or CD5 (Ly-1) MicroBeads.
CD49b is less mouse strain–specific than other NK cell markers (e.g. NK1.1) and is expressed by the most common inbred mouse strains.

Downstream applications

NK cells isolated with CD49b (DX5) MicroBeads from wild-type or immunodeficient mice were used to study NK cell cytotoxicity in vitro1 or after adoptive transfer of isolated NK cells in vivo2. Furthermore, isolated NK cells were used to evaluate their role in experimental mouse models of immune-mediated liver injury3 and chronic infection4.


For positive selection: MS, LS, XS, or autoMACS® Columns. For depletion: LD, CS, D, or autoMACS Columns.


Figure 1

Figure 1
Isolation of mouse NK cells from a mouse spleen cell suspension using CD49b (DX5) MicroBeads, a MiniMACS™ Separator, and an MS Column.
Isolation of mouse NK cells from a mouse spleen cell suspension using CD49b (DX5) MicroBeads, a MiniMACS™ Separator, and an MS Column.

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Selected references

  1. Tomasello, E. et al. (2000) Combined natural killer cell and dendritic cell functional deficiency in KARAP/DAP12 loss-of-function mutant mice. Immunity 13: 355–364.
  2. Shi, F. D. et al. (2000) IL-18 directs autoreactive T cells and promotes autodestruction in the central nervous system via induction of IFN-gamma by NK cells. J. Immunol. 165: 3099–3104.
  3. Mühlen, K. A. et al. (2004) NK cells, but not NKT cells, are involved in Pseudomonas aeruginosa exotoxin A-induced hepatotoxicity in mice. J. Immunol. 172: 3034–3041.
  4. Hsieh, G. C. et al. (2004) A secreted protein from the human hookworm necator americanus binds selectively to NK cells and induces IFN-gamma production. J. Immunol. 173: 2699–2704.

Customer reports and application notes

Product Order no. Price

CD49b (DX5) MicroBeads, mouse

Capacity: for 2×109 total cells
Data sheet
130-052-501 $640.00

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Miltenyi Biotec Inc.
Phone: +1 800 FOR MACS
Fax:+1 877 591 1060
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