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CD16 MicroBeads, non-human primate

Overview

CD16 MicroBeads have been developed for the positive selection or depletion of NK cells from rhesus monkey PBMCs. CD16 is expressed on the majority of rhesus monkey NK cells1 and on a subset of monocytes. Monocytes can be depleted prior to separation using CD56 MicroBeads or CD14 MicroBeads, respectively.

Details

Downstream applications

CD16+ NK cells can, for example, be used for studies on NK cell cytotoxicity or signal transduction.

Columns

For positive selection: MS, LS, XS, or autoMACS® Columns. For depletion: LD, CS, D, or autoMACS Columns.

Gallery

Figure 1

Positive selection of CD16+ NK cells from rhesus monkey PBMCs using CD16 MicroBeads, a MiniMACS™ Separator, and an MS Column. As some rhesus monkey monocytes express CD16, monocytes1 as well as a minor subset of CD56+ NK cells2 were depleted by using CD56 MicroBeads prior to positive selection of CD16+ cells.
PBMCs after depletion of CD56+ cells
CD16+ NK cells

Related Items

Library

Selected references

  1. Carter, D. L. et al. (1999) CD56 identifies monocytes and not natural killer cells in Rhesus macaques. Cytometry 37: 41–50.
  2. Webster, R. L. and Johnson, R. P. (2005) Delineation of multiple subpopulations of natural killer cells in rhesus macaques. Immunology 115: 206–214.

Product Order no. Price

CD16 MicroBeads, non-human primate

Capacity: for 1×109 total cells
 
Data sheet
130-091-145 $630.00

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Miltenyi Biotec Inc.
Phone: +1 800 FOR MACS
Fax:+1 877 591 1060
macs@miltenyibiotec.com
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