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Anti-iNKT MicroBeads, human

Overview

Anti-iNKT MicroBeads have been developed for the positive selection of human invariant natural killer T cells (iNKT cells) based on the expression of the TCR α-chain Vα24-Jα18.

Details

Background information

iNKT cells represent a distinct lymphocyte population, characterized by expression of an invariant T cell receptor α-chain and certain TCR β-chains (Vα24-Jα18 combined with Vβ11). iNKT cells are involved in immunoregulatory processes such as tolerance, host defense, and tumor surveillance.
Due to the nature of the antibody, a limited degree of pre-activation of target cells might occur after separation with this product.

Columns

MS, LS, XS, or autoMACS® Columns.

Gallery

Figure 1

The iNKT cells were isolated from human PBMCs using Anti-iNKT MicroBeads, two MS Columns, and a MiniMACS™ Separator. Cells were fluorescently stained with Anti-iNKT-PE, CD3-APC, and CD45-FITC and analyzed using the MACSQuant® Analyzer. Gating was performed according to CD45 expression and side scatter properties of the cells.
Unseparated fraction
After separation

Related Items

Library

Selected references

  1. Exley, M. et al. (2008) Selective activation, expansion, and monitoring of human iNKT cells with a monoclonal antibody specific for the TCR α-chain CDR3 loop. Eur. J. Immunol. 38: 1756–1766.
  2. Montoya, J.C. et al. (2007) Characterization of human invariant natural killer T subsets in health and disease using a novel invariant natural killer T cell-clonotypic monoclonal antibody, 6B11. Immunology 122: 1–14.

Product Order no. Price

Anti-iNKT MicroBeads, human

Capacity: for 2×109 total cells
 
Data sheet
130-094-842 $650.00

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Miltenyi Biotec Inc.
Phone: +1 800 FOR MACS
Fax:+1 877 591 1060
macs@miltenyibiotec.com
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