The Classical Monocyte Isolation Kit provides fast and consistent isolation of untouched classical ( CD14++CD16–) monocytes from peripheral blood mononuclear cells (PBMCs) for further functional studies. Depletion of non-monocytes is optimal and provides a highly pure population of label-free classical monocytes. This product includes an optional thrombocytes removal reagent to further reduce platelet contamination.
Non-monocytes, such as T cells, NK cells, B cells, dendritic cells, and basophils are indirectly magnetically labeled using a cocktail of biotin-conjugated antibodies and Anti-Biotin MicroBeads. Isolation of highly pure unlabeled monocytes is achieved by depletion of the magnetically labeled cells.
Downstream applicationsFunctional studies on monocytes, in which any effect due to antibody binding to target cells should be avoided.
Studies on monocyte activation, differentiation, cytokine secretion etc.
Studies on compound or antigen uptake (e.g. phagocytosis) and antigen presentation by monocytes.
In vitro differentiation of blood monocytes into dendritic cells or macrophages.
MS Columns, LS Columns, autoMACS® Columns, or Multi-24 Column Blocks.
CD14+CD16– cells were isolated from human peripheral blood mononuclear cells (PBMCs) by using the Classical Monocyte Isolation Kit, an LS Column, and a MidiMACS™ Separator. The cells were fluorescently stained with CD14-FITC and CD16-APC and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.