Products and Services - MACS Cell Separation - Cell separation reagents - MicroBeads and Isolation Kits - Hematopoietic stem cells - CD117 MicroBeads, mouse

CD117 MicroBeads, mouse


CD117 MicroBeads have been developed for the positive selection of hematopoietic stem and progenitor cells from mouse bone marrow . In addition, CD117 MicroBeads have been successfully used for the isolation of a variety of tissue stem cells, e.g. differentiated embryonic stem cell cultures1, or amniotic fluid-derived stem cells2.


Background information

CD117 (c-kit) is a receptor tyrosine kinase expressed on the majority of hematopoietic stem and progenitor cells, on some tissue stem cells as well as mature mast cells. Stimulation of CD117 by its ligand, the cytokine stem cell factor (SCF), is important for stem cell survival and maintance.

Downstream applications

  • Further flow cytometric analysis or sorting of isolated CD117+ cells, e.g. into LSK populations3.
  • Transplantation of CD117+ bone marrow cells, e.g. after myocardial infarction for differentiation into smooth muscle cells, myocytes, and endothelial cells4.


MS, LS, XS, or autoMACS® Columns.


Figure 1

Separation of bone marrow cells using CD117 MicroBeads, a MiniMACS™ Separator, and an MS Column. Cells were fluorescently stained with lineage markers (biotinylated antibodies against CD5, CD11b, CD45R, Gr-1, 7-4, Ter-119 and Anti-Biotin-APC) and CD117-PE.
A: Before enrichment
B: Isolated CD117+ cells after enrichment

Related Items


Selected references

  1. Verda, L. et al. (2008) Hematopoietic mixed chimerism derived from allogeneic embryonic stem cells prevents autoimmune diabetes mellitus in NOD mice. Stem Cells 26: 381–386.
  2. De Coppi, P. et al. (2007) Isolation of amniotic stem cell lines with potential for therapy. Nat. Biotechnol. 25: 100–106.
  3. Shin, J.Y. et al. (2014) High c-Kit expression identifies hematopoietic stem cells with impaired self-renewal and megakaryocytic bias J. Exp. Med. 211(2): 217–231.
  4. Orlic, D. et al. (2001) Mobilized bone marrow cells repair the infarcted heart, improving function and survival. Proc. Natl. Acad. Sci. U.S.A. 98: 10344–10349.

Product Order no. Price

CD117 MicroBeads, mouse

Capacity: for 1×109 total cells
Data sheet
130-091-224 $555.00

CD117 MicroBeads, mouse – lyophilized

Capacity: for 1×109 total cells
Data sheet
130-097-146 $555.00

Customer login


Forgot password?
Register for account

0 Shopping cart


Miltenyi Biotec Inc.
Phone: +1 800 FOR MACS
Fax:+1 877 591 1060
Contact overview

Product finder

Antibody panel builder



Get rewarded for the reagents you love

Get rewarded for reagents

Change country

Please select your country to continue