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Anti-A2B5 MicroBead Kit, human and mouse

Overview

The Anti-A2B5 MicroBead Kit has been developed for the positive selection of human and mouse pluripotent stem cell-derived glial precursor cells based on the expression of the A2B5 antigen.

Details

Background information

The Anti-A2B5 MicroBead Kit has been developed for the positive selection of human and mouse pluripotent stem cell-derived glial precursor cells based on the expression of the A2B5 antigen.
For isolation of primary A2B5+ cells please refer to Anti-A2B5 Microbeads.
Ganglioside GT3 and its O-acetylated derivative are the principal A2B5-reactive gangliosides. In vitro differentiation of human and mouse pluripotent stem cells under defined conditions revealed that oligodendrocyte precursor cells express the surface marker A2B5. Magnetic enrichment of in vitro generated glial precursors will facilitate access to a highly pure A2B5-positive cell population and may even shorten established differentiation protocols.

Gallery

Figure 1

A2B5+ cells were isolated from human pluripotent stem cell-derived glial precursor cells using the Anti-A2B5 MicroBead Kit, an LS Column, and a MiniMACS™ Separator. Cells were analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Before separation
A2B5+ cells

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Selected references

  1. Barral, S. et al. (2013) Efficient neuronal in vitro and in vivo differentiation after immunomagnetic purification of mESC derived neuronal precursors. Stem Cell Res. 10(2): 133–46.

Product Order no. Price

Anti-A2B5 MicroBead Kit, human and mouse

Capacity: for 1×109 total cells
 
Data sheet
130-097-864 $600.00

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Miltenyi Biotec Inc.
Phone: +1 800 FOR MACS
Fax:+1 877 591 1060
macs@miltenyibiotec.com
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