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Anti-PE MicroBeads

Overview

Anti-Phycoerythrin (PE) MicroBeads are used for the indirect magnetic labeling and separation of cells or other materials labeled with a PE-conjugated primary antibody or ligand. The use of PE-conjugated primary antibodies and Anti-PE MicroBeads leads to the highest purity and recovery, even when the marker used for sorting is weakly expressed.

Details

Background information

The PE molecule is recognized by the monoclonal anti-PE antibody coupled to MicroBeads, allowing the separation of the fluorescently labeled material. Subsequent flow cytometric analysis is possible without any further staining.

Applications

Anti-PE MicroBeads are well-established as a component of the Cytokine Secretion Assays for the enrichment of antigen-specific T cells. They have also been used to isolate antigen-specific T cells after labeling with PE-conjugated MHC Class I tetramers1,2 or MHC Class II tetramers3. Moreover, they have been used to separate a CD62L+ subset of CD4+CD45RO+ memory T cells from human adenoids, which had been pre-sorted by MACS® Technology4.

Columns

For positive selection: MS, LS, XS, or autoMACS® Columns. For depletion: LD, CS, D, or autoMACS Columns.

Gallery

Figure 1

Isolation of cytomegalovirus (CMV)-specific T cells from PBMCs using PE-conjugated CMVpp65/A2 tetramers (courtesy of Prof. Moss, Birmingham, U.K.) and Anti-PE MicroBeads. Cells were positively selected by separation over two MS Columns.
PBMCs before separation
Enriched CMV-specific T cells

Library

Selected references

  1. Shankar et al. (2001) Blood 96: 3094–3101.
  2. Barnes et al. (2004) Eur. J. Immunol. 34: 1570–1577.
  3. Lucas et al. (2004) J. Virol. 78: 7284–7287.
  4. Mattila et al. (2000) Int. Immunol. 12: 1235–1243.

Product Order no. Price

Anti-PE MicroBeads

Capacity: for 1×109 total cells
 
Data sheet
130-048-801 $525.00

Anti-PE MicroBeads – lyophilized

Capacity: for 1×109 total cells
 
Data sheet
130-097-054 $525.00

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Miltenyi Biotec Inc.
Phone: +1 800 FOR MACS
Fax:+1 530 745 2806
macs@miltenyibiotec.com
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