Anti-Phycoerythrin (PE) MicroBeads are used for the indirect magnetic labeling and separation of cells or other materials labeled with a PE-conjugated primary antibody or ligand. The use of PE-conjugated primary antibodies and Anti-PE MicroBeads leads to the highest purity and recovery, even when the marker used for sorting is weakly expressed.
The PE molecule is recognized by the monoclonal anti-PE antibody coupled to MicroBeads, allowing the separation of the fluorescently labeled material. Subsequent flow cytometric analysis is possible without any further staining.
Anti-PE MicroBeads are well-established as a component of the Cytokine Secretion Assays for the enrichment of antigen-specific T cells. They have also been used to isolate antigen-specific T cells after labeling with PE-conjugated MHC Class I tetramers1,2 or MHC Class II tetramers3. Moreover, they have been used to separate a CD62L+ subset of CD4+CD45RO+ memory T cells from human adenoids, which had been pre-sorted by MACS® Technology4.
For positive selection: MS, LS, XS, or autoMACS® Columns. For depletion: LD, CS, D, or autoMACS Columns.
Isolation of cytomegalovirus (CMV)-specific T cells from PBMCs using PE-conjugated CMVpp65/A2 tetramers (courtesy of Prof. Moss, Birmingham, U.K.) and Anti-PE MicroBeads. Cells were positively selected by separation over two MS Columns.
PBMCs before separation
Enriched CMV-specific T cells