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Anti-PE MicroBeads

Overview

Anti-Phycoerythrin (PE) MicroBeads are used for the indirect magnetic labeling and separation of cells or other materials labeled with a PE-conjugated primary antibody or ligand. The use of PE-conjugated primary antibodies and Anti-PE MicroBeads leads to the highest purity and recovery, even when the marker used for sorting is weakly expressed. Anti-PE MicroBeads UltraPure have been especially designed for cell separations from debris-rich biological starting materials.

Details

Background information

Anti-PE MicroBeads UltraPure have been especially developed for highly efficient separation of cells from debris-rich samples or other biological materials according to surface markers labeled with PE-conjugated primary antibodies, peptides or ligands. After separation the PE-labeled cells can be directly detected by flow cytometry or fluorescence microscopy without any further staining. Together with its ultrapure cell separation result even from debris-rich starting material Anti-PE MicroBeads UltraPure are the latest development of Anti-PE MicroBeads.

Applications

Anti-PE MicroBeads are well-established as a component of the Cytokine Secretion Assays for the enrichment of antigen-specific T cells. They have also been used to isolate antigen-specific T cells after labeling with PE-conjugated MHC Class I tetramers1,2 or MHC Class II tetramers3. Moreover, they have been used to separate a CD62L+ subset of CD4+CD45RO+ memory T cells from human adenoids, which had been pre-sorted by MACS® Technology4. The latest development of Anti-PE MicroBeads UltraPure enables high-yield positive cell separation using any PE-labeled primary antibody, even from low quality and rare cell samples.

Columns

For positive selection: MS, LS, XS, or autoMACS® Columns. For depletion: LD, CS, D, or autoMACS Columns.

Gallery

Figure 1

Isolation of cytomegalovirus (CMV)-specific T cells from PBMCs using PE-conjugated CMVpp65/A2 tetramers (courtesy of Prof. Moss, Birmingham, U.K.) and Anti-PE MicroBeads. Cells were positively selected by separation over two MS Columns.
PBMCs before separation
Enriched CMV-specific T cells

Figure 2

Human CLEC9a+ cells have been isolated from a debris-rich sample of peripheral blood mononuclear cells (PBMCs) using Anti-CLEC9a-PE, Anti-PE MicroBeads UltraPure, two MS Columns, and a MiniMACS™ Separator. Cells were stained with Anti-CLEC9a-PE (# 130-097-368), CD141 (BDCA-3)-APC (# 130-090-907), and Propidium Iodide Solution (# 130-093-233). Cells were analyzed after gating on viable lymphoid cells.
Before separation
After separation

Related Items

Library

Selected references

  1. Shankar et al. (2001) Blood 96: 3094–3101.
  2. Barnes et al. (2004) Eur. J. Immunol. 34: 1570–1577.
  3. Lucas et al. (2004) J. Virol. 78: 7284–7287.
  4. Mattila et al. (2000) Int. Immunol. 12: 1235–1243.

Product Order no. Price

Anti-PE MicroBeads

Capacity: for 1×109 total cells
 
Data sheet
130-048-801 $525.00

Anti-PE MicroBeads – lyophilized

Capacity: for 1×109 total cells
 
Data sheet
130-097-054 $525.00

Anti-PE MicroBeads UltraPure

Capacity: for 1×109 total cells
 
Data sheet
130-105-639 $550.00

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Miltenyi Biotec Inc.
Phone: +1 800 FOR MACS
Fax:+1 530 745 2806
macs@miltenyibiotec.com
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