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autoMACS® Pro Separator - Starter Kit

Overview

The autoMACS® Pro Separator is a benchtop instrument for high-speed magnetic cell sorting of multiple samples. Employing MACS® Technology, the autoMACS Pro Separator is designed for cell isolation in a fully automated, walk-away fashion.

Details

The autoMACS Pro Separator combines all the benefits of the proven autoMACS Technology with novel features such as automated magnetic labeling and sensor-controlled processing of multiple samples. The instrument status can be viewed from a distance, facilitated by illumination of buffer bottles by light-emitting diodes. The instrument is operated via touchscreen and intuitive screen menus. At the touch of a button, multisample labeling and cell separation are performed, minimizing hands-on time. Sample integrity is also maintained by use of MACS Chill Racks that allow for cooling of samples and eluted fractions. With its compact size, the autoMACS Pro Separator easily fits into a standard laminar flow hood or safety cabinet.

Applications

The autoMACS Pro Separator is compatible with more than 250 MACS Cell Separation Reagents for easy and fast isolation of virtually any cell type from any species.
Furthermore, the autoMACS Pro Separator enables cell sorting directly from whole blood or bone marrow without the need for density-gradient centrifugation or erythrocyte lysis.
The compatibility of MACS Technology with flow cytometry allows the use of the autoMACS Pro Separator as an alternative to flow sorting. Alternatively, the autoMACS Pro Separator can be used as a pre-sorter for speeding up flow sorting.

Gallery

Figure 1

Figure 1
autoMACS® Pro Separator
autoMACS® Pro Separator

Miltenyi TV

Video 1

Immediate delivery
Walkaway multisampling on the autoMACS Separator

Walkaway multisampling on the autoMACS Separator

The MiniSampler on the autoMACS Pro Separator allows up to 6 samples to be processed in a single batch - from rare cell isolation to isolation of abundant cells.

Video 2

Immediate delivery
Autolabeling on the autoMACS Pro Separator

Autolabeling on the autoMACS Pro Separator

The autoMACS Pro Separator performs fully automated sample labeling in a reliable and reproducible fashion. This offers you the convenience of walkaway sample processing. The autolabeling feature ensures reproducibility and consistent results.

Video 3

Immediate delivery
Cell separation from whole blood with the autoMACS Pro Separator.

Cell separation from whole blood with the autoMACS Pro Separator.

With the autoMACS Pro Separator and Whole Blood MicroBeads it is possible to isolate pure cell populations of interest from whole blood or bone marrow samples very easily within 30 minutes.

Related Items

Library

Selected references

  1. Kulis, M. et al. (2015) Whole‑genome fingerprint of the DNA methylome during human B cell differentiation. Nat. Genet. 47(7): 746–756.
  2. Haus, D. L. et al. (2014) CD133-enriched Xeno-Free human embryonic-derived neural stem cells expand rapidly in culture and do not form teratomas in immunodeficient mice Stem Cell Res. 13(2): 214–226.
  3. Kim, MS. et al. (2014) A draft map of the human proteome Nature 509(7502): 575–581.
  4. Kuwahara, M. et al. (2014) The Menin-Bach2 axis is critical for regulating CD4 T-cell senescence and cytokine homeostasis Nat Commun 2(5): 3555.
  5. Riquelme, P. et al. (2013) IFN-γ-induced iNOS expression in mouse regulatory macrophages prolongs allograft survival in fully immunocompetent recipients Mol. Ther. 21(2): 409–422.
  6. Bénéteau, M. et al. (2012) Combination of glycolysis inhibition with chemotherapy results in an antitumor immune response Proc. Natl. Acad. Sci. U.S.A. 109(49): 20071–20076.
  7. Carlson, S. et al. (2011) Cardiac mesenchymal stem cells contribute to scar formation after myocardial infarction Cardiovasc. Res. 91: 99–107.
  8. Liu, Y. et al. (2011) Expression of p16INK4a prevents cancer and promotes aging in lymphocytes Blood 117: 3257–3267.
  9. Perez, J. et al. (2011) Accumulation of CD4+ T cells in the colon of CsA-treated mice following myeloablative conditioning and bone marrow transplantation Am. J. Physiol. Gastrointest. Liver Physiol. 300: G843–G852.
  10. Vargova, K. et al. (2011) MYB transcriptionally regulates the miR-155 host gene in chronic lymphocytic leukemia. Blood 2011(117): 3816–3825.
  11. Willasch et al. (2010) Enrichment of cell subpopulations applying automated MACS technique: purity, recovery and applicability for PCR-based chimerism analysis Bone Marrow Transplant. 45: 181–189.
  12. Lindstrom D.L. and Gottschling D.E. (2009) The mother enrichment program: a genetic system for facile replicative life span analysis in Saccharomyces cerevisiae Genetics 183(2): 413–422.

Brochures and posters

Customer reports and application notes

autoMACS Pro vs. FACS [PDF, 809 kB, EN]

Further downloads

Product Order no. Price

autoMACS Pro Separator - Starter Kit

Components:
  • autoMACS Pro Separator Instrument
  • MACS MiniSampler
  • MACS Reagent Rack 4 (130-094-574)
  • Chill 5, 15, 50 Set (130-097-038)
  • barcode reader
  • software
  • autoMACS® Columns (130-021-101)
  • 4×column substitute
  • autoMACS Pro Buffer Combination
  • User manual
  • short instructions
  • one-year warranty
 
User manual
130-092-545 price on request here

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Miltenyi Biotec Inc.
Phone: +1 800 FOR MACS
Fax:+1 877 591 1060
macs@miltenyibiotec.com
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