Products and Services - MACS Cell Separation - Automated cell separation - autoMACS Pro Separator

Fully automated cell separation: autoMACS® Pro Separator

  • True walk-away cell isolation with automated labeling and separation
  • Standardized cell separation for reproducible, user-independent results
  • Fast and gentle isolation of virtually any cell type from any species

With the autoMACS® Pro Separator you can isolate cells fully automated, directly from whole blood or bone marrow, which makes it ideal for multiple myeloma research or lineage-specific chimersim analysis after bone marrow transplantation. autoMACS technology hast been cited in over 2300 peer-reviewed publications, making it the most trusted, most proven automated magnetic cell separation technology.


The gold standard in automated cell separation

The autoMACS Pro Separator is compatible with more than 350 MACS Cell Separation Reagents for an easy and fast isolation of virtually any cell type from any species. The unique features of MACS MicroBead Technology qualify the autoMACS® Pro Separator as an alternative or complement to flow sorting and allow for a broad range of downstream applications such as flow cytometry , cell culture , functional assays , FISH , PCR  and omic approaches 

Explore the autoMACS® Pro Separator features by clicking on the orange buttons: 

1. Smart sensor-controlled process:
The fully-automated sensor-controlled process assures the safety of your precious samples.
  • Bottle illumination indicates instrument status, observable from across the laboratory
  • Monitoring of buffer fluid levels
  • Monitoring of column status
  • Automatic detection of hardware peripherals
2. MACS Chill Racks
Maintain the integrity of your valuable samples by keeping your cells at 4° C.
  • Walk-away cell separation - Chill Racks allow up to 6 samples to be processed in a single batch
  • Flexible format, available for use with 50 mL, 15 mL or 5 mL tubes
3. MACS MiniSampler
Smart tools for smart walk-away cell separation.
  • The MACS MiniSampler accommodates one MACS Chill Rack and one MACS Reagent Rack
  • The MACS Reagent Rack holds up to four reagent vials for multisample autolabeling
4.Autolabeling of multiple samples
Extremely convenient walk-away cell separations, while ensuring the consistency of your results.
  • Achieve reproducibility every time, no expertise required
  • Hands-free sample processing leads to safe laboratory practices: of vital importance when dealing with hazardous samples
5. Columns
One column for all automated cell separation applications.
  • Can be used for up to 14 days or 100 separations within 14 days
  • Automatic detection, monitoring, and priming of column managed by instrument software
6. MACS Buffers
Peace of mind with MACS Buffers.
  • Efficient cleaning between cell separations: the autoMACS® Pro Washing Solution has been developed for rinsing and cleaning of the fluidic system
  • Ready to use: MACS Buffers are optimized and quality-controlled for automated cell separation requirements
  • High-quality ingredients and sterile filtered


The autoMACS Columns are specifically designed for use with the autoMACS Pro Separator. A set of two separation columns is required for operating the instrument. The columns can be used for up to 14 days or 100 separations within 14 days.

Sorting from whole blood

Isolate cells directly from whole blood or bone marrow while avoiding red blood cell lysis and density-gradient centrifugation steps.
The Whole Blood MicroBeads are simply added to blood samples treated with anticoagulant; leukocyte subsets are subsequently purified by using the autoMACS® Pro Separator.

  • No density-gradient centrifugation
  • Minimized cell loss, optimal for small blood volumes
  • Easy and safe handling of hazardous samples
The purified cells are well-suited for further flow cytometric analysis, molecular biology studies, such as lineage-specific chimerism analysis, and functional studies.
Whole Blood MicroBeads are available for CD3+, CD4+, CD8+, CD14+, CD15+, CD19+, CD33+, CD45+, CD56+, or CD138+ cells.

Demo / Quote request

Our worldwide sales and technical support teams are available to assist you with any questions you may have about cell separation and related instrumentation. To ask for more information, or request a demonstration, simply complete the online form.
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autoMACS Pro Separator - Starter Kit

  • autoMACS Pro Separator Instrument
  • MACS MiniSampler
  • MACS Reagent Rack 4 (130-094-574)
  • Chill 5, 15, 50 Set (130-097-038)
  • barcode reader
  • software
  • autoMACS® Columns (130-021-101)
  • 4×column substitute
  • autoMACS Pro Buffer Combination
  • User manual
  • short instructions
  • one-year warranty
130-092-545 price on request here

autoMACS® Columns

Content: 5×2 columns
130-021-101 $685.00
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Miltenyi Biotec Inc.
Phone: +1 800 FOR MACS
Fax:+1 877 591 1060
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