Products and Services - MACS Cell Culture and Stimulation - Stimulation reagents - Peptide pools - PepTivator BKV LT, human

PepTivator BKV LT, human


PepTivator® BKV LT is a pool of lyophilized peptides, consisting mainly of 15-mer sequences with 11 amino acids overlap, covering the complete sequence of the human BK virus (BKV) large T (LT) antigen (UniProt ID: P14999).
The in vitro stimulation of antigen-specific T cells with PepTivator Peptide Pools causes the secretion of effector cytokines and the up-regulation of activation markers, which then allow the detection and isolation of antigen-specific T cells.


Background information

BKV is a ubiquitous polyomavirus. After primary infection BKV persists in a latent state. Virus reactivation might occur in immunodeficiency or during immunosuppression. The LT antigen is expressed early in the lytic cycle and is involved in virus replication.

Downstream applications

PepTivator BKV LT - research grade has been specifically developed for efficient in vitro stimulation of BKV LT–specific T cells. Peptides of 15 amino acids in length and 11 amino acids overlap represent an optimized solution for stimulating both CD4+ and CD8+ T cells in various applications, including:
  • Detection and analysis of BKV LT–specific effector/memory T cells in PBMCs by MACS® Cytokine Secretion Assays, intracellular cytokine staining, or other technologies.
  • Isolation of viable BKV LT–specific CD4+ T cells with the CD154 MicroBead Kit, or of CD4+ and CD8+ T cells using the CD137 MicroBead Kit or MACS Cytokine Secretion Assay – Cell Enrichment and Detection Kits. Subsequently, cells can be expanded for generation of T cell lines.
  • Generation of BKV LT–specific effector/memory T cells from naive T cell populations.
  • Pulsing of antigen-presenting cells.


Figure 1

Detection and isolation of viable BKV–specific T cells using MACS® IFN-γ Secretion Assay – Cell Enrichment and Detection Kit (PE)
From a BKV+ donor, 107 human peripheral blood mononuclear cells (PBMCs) were restimulated for 4 hours either with 20 μL/mL of reconstituted PepTivator® BKV LT1 (A), 20 µL/mL BKV VP1 (B), or a mixture of both peptide pools (C). BKV-specific T cells were stained and magnetically enriched according to their secretion of IFN-γ using the IFN-γ Secretion Assay – Cell Enrichment and Detection Kit (PE) (# 130-054-201). T cells were counterstained for CD4 and CD8 expression. Cell debris and dead cells are excluded from the analysis based on scatter signals and propidium iodide fluorescence. IFN-γ secretion of viable lymphocytes is shown.
A: Unstimulated
Before enrichment
After enrichment
B: Unstimulated
Before enrichment
After enrichment
C: Unstimulated
Before enrichment
After enrichment

Related Items

Product Order no. Price

PepTivator BKV LT – research grade, human

Capacity: for stimulation of 1×108 cells
Content: 6 nmol/peptide
Data sheet
130-096-504 $170.00

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