Products and Services - MACS Cell Culture and Stimulation - Stimulation reagents - Functional-grade antibodies - Anti-IFN-γ antibodies – functional grade, human

Anti-IFN-γ antibodies – functional grade, human (clone: 45-15)


Interferon gamma (IFN-γ) is produced by cells that are involved in inflammatory immune responses. IFN-γ is predominantly released by memory and effector CD4+ and CD8+ T cells as well as by NK cells upon activation. Quantitative analysis of IFN-γ–producing cells can provide important information on the course of immune responses. Anti-IFN‑γ antibodies are designed for intracellular staining of IFN‑γ–producing cells. Cells can be stimulated for IFN-γ–production, e.g. by polyclonal stimulation with mitogens. For induction of IFN‑γ–production by antigen-specific T cells, cells are restimulated with respective antigen. IFN‑γ can be accumulated in the cells by addition of secretion inhibitors like brefeldin A. After fixation and permeabilization of the cell sample, IFN‑γ–producing cells can be stained intracellularly with Anti-IFN‑γ antibodies. Staining of surface markers allows simultaneous flow cytometric analysis of subsets and activation status of the IFN-γ–producing cells.


The Anti-IFN-γ pure – functional grade antibody is suited for functional assays, for example, neutralization of IFN-γ activity, in vitro inhibition of Tʜ1 cell polarization, and promotion of Tʜ2 cell and Tʜ17 cell polarization.

Technical data

Antigen IFN-γ
Clone 45-15
Isotype mouse IgG1
Isotype control
Alternative names of antigen IFNg, IFG, IFI
Entrez Gene ID 3458
Molecular mass of antigen [kDa] 16
Cross-reactivity rhesus monkey (Macaca mulatta), cynomolgus monkey (Macaca fascicularis)
Distribution of antigen
Endotoxin level
Endotoxin levels have been tested and do not exceed 0.01 ng/µg of protein.
Product format Antibodies are supplied in phosphate-buffered saline (PBS), pH 7.2. The product contains no preservative and is sterile filtered. Always handle under aseptic conditions.
Storage Store protected from light at 2–8 °C. Do not freeze.
Available conjugates pure

Related Items

Product Order no. Price

Anti-IFN-γ pure – functional grade, human (clone: 45-15)

For research use only
Content: 500 µg in 0.5 mL
Data sheet
130-095-743 $360.00

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