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Anti-FcεRIα antibodies – functional grade, human (clone: CRA1)

Overview

Clone CRA1 detects the alpha subunit of the human high-affinity Fc receptor for IgE. The receptor is composed of one alpha, one beta and two disulfid-linked gamma subunits, of which the alpha subunit binds IgE1. FcεRIα is expressed on mast and basophilic cells and is up-regulated in the presence of IgE2,3. The FcεRI complex plays an important role in triggering IgE-mediated allergic reactions. Antibody clone CRA1 does not inhibit binding of IgE4. It induces mast cell activation, followed by histamin release5.

Applications

This pure - functional grade antibody is suited for cell culture functional assays, for example, blocking of receptor-ligand binding.

Technical data


Antigen FcεRIα
Clone CRA1
Isotype mouse IgG2b
Isotype control
Alternative names of antigen FCER1A, Fce1a, FcERI
Entrez Gene ID 2205
Molecular mass of antigen [kDa] 27
Cross-reactivity
Distribution of antigen
Endotoxin level
Endotoxin levels have been tested and do not exceed 0.01 ng/µg of protein.
Product format Antibodies are supplied in phosphate-buffered saline (PBS), pH 7.2. The product contains no preservative and is sterile filtered. Always handle under aseptic conditions.
Fixation
Storage Store protected from light at 2–8 °C. Do not freeze.
Available conjugates pure

Related Items

Library

Selected references

  1. Hasegawa, S. et al. (1999) Functional expression of the high affinity receptor for IgE (FcepsilonRI) in human platelets and its`intracellular expression in human megakaryocytes. Blood 93: 2543–2551.
  2. Suzukawa, M. et al. (2005) IgE- and FcepsilonRI-mediated migration of human basophils. Int. Immunol. 17: 1249–1255.
  3. Hakimi, J. et al. (1990) The alpha subunit of the human IgE receptor (FcERI) is sufficient for high affinity IgE binding. J. Biol. Chem. 265: 22079–22081.
  4. Takai, T. et al. (2000) Epitope analysis and primary structures of variable regions of anti-human FcepsilonRI monoclonal antibodies, and expression of the chimeric antibodies fused with human constant regions. Biosci. Biotechnol. Biochem. 64: 1856–1867.
  5. Yokoi, H. et al. (2008) Inhibition of Fcepsilon RI-dependent mediator release and calcium flux from human mast cells by sialic acid-binding immunoglobulin-like lectin 8 engagement. J. Allergy Clin. Immunol. 121: 499–505.

Product Order no. Price

Anti-FcεRIα pure – functional grade, human (clone: CRA1)

For research use only
Content: 100 µg in 1 mL
 
Data sheet
130-096-004 $150.00

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Miltenyi Biotec Inc.
Phone: +1 800 FOR MACS
Fax:+1 877 591 1060
macs@miltenyibiotec.com
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