AstroMACS Medium - serum free

Save 50% on your first bottle of serum-free astrocyte culture medium now

Are you looking for serum-free medium which also supports excellent astrocyte growth in vitro? Or do you want to culture astrocytes from adult mouse brain? Then we have the right products for you!

Our serum-free AstroMACS Medium supports

  • Superior neonatal astrocyte growth even with very low seeding densities
  • Enhanced adult astrocyte culture together with the new AstroMACS Separation Buffer, which removes dead cells during cell separation and provides a healthy culture environment

Superior astrocyte growth even with low seeding densities

Neonatal astrocytes were isolated from P4 mice using the Neural Tissue Dissociation Kit (P) and the Anti-ACSA-2 MicroBead Kit. Cells were cultured in MACS® Neuro Medium and MACS NeuroBrew®-21 (top) or in AstroMACS Medium (bottom) at different densities in a 24-well imaging plate for 7 days. Cells were fixed and stained with Anti-GLAST antibody (yellow) and DAPI (blue) and dead and viable astrocytes were analyzed with the Cytation™ Cell Imaging Multi-Mode Reader. (A) Microscopy images of one representative well. (B) Quantitative data of 4 replicates.
Neonatal astrocytes were isolated from P4 mice using the Neural Tissue Dissociation Kit (P) and the Anti-ACSA-2 MicroBead Kit. Cells were cultured in MACS® Neuro Medium and MACS NeuroBrew®-21 (top) or in AstroMACS Medium (bottom) at different densities in a 24-well imaging plate for 7 days. Cells were fixed and stained with Anti-GLAST antibody (yellow) and DAPI (blue) and dead and viable astrocytes were analyzed with the Cytation™ Cell Imaging Multi-Mode Reader. (A) Microscopy images of one representative well. (B) Quantitative data of 4 replicates.

AstroMACS Separation Buffer removes dead cells and enhances adult astrocyte culture

Adult astrocytes were isolated from the brain of 8-week-old mice using the Adult Brain Dissociation Kit and the Anti-ACSA-2 MicroBead Kit in combination with (A) or without (B) the AstroMACS Separation Buffer. Cells were then cultivated in AstroMACS Medium at a density of 100,000 cells /well in a 24-well Imaging Plate for 7 days. Cells were fixed and stained with Anti-GLAST antibody (green) and DAPI (blue).
Adult astrocytes were isolated from the brain of 8-week-old mice using the Adult Brain Dissociation Kit and the Anti-ACSA-2 MicroBead Kit in combination with (A) or without (B) the AstroMACS Separation Buffer. Cells were then cultivated in AstroMACS Medium at a density of 100,000 cells /well in a 24-well Imaging Plate for 7 days. Cells were fixed and stained with Anti-GLAST antibody (green) and DAPI (blue).

Preservation of healthy astrocytes for reliable downstream experiments

Neonatal astrocytes were isolated from P4 mice using the Neural Tissue Dissociation Kit (P) and the Anti-ACSA-2 MicroBead Kit, and cultured in AstroMACS Medium at a density of 10,000 cells / well in a 24-well imaging plate for 7 days. Cells were then fixed and stained with Anti-GLAST antibody (green) and DAPI (blue).
Neonatal astrocytes were isolated from P4 mice using the Neural Tissue Dissociation Kit (P) and the Anti-ACSA-2 MicroBead Kit, and cultured in AstroMACS Medium at a density of 10,000 cells / well in a 24-well imaging plate for 7 days. Cells were then fixed and stained with Anti-GLAST antibody (green) and DAPI (blue).

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Miltenyi Biotec Inc.
Phone: +1 800 FOR MACS
Fax:+1 877 591 1060
macs@miltenyibiotec.com
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