Clinical applications - MACS GMP Products - Cytokines - MACS GMP Recombinant Human IL-6

MACS GMP Recombinant Human IL-6

Overview

Interleukin 6 (IL-6) is a multifunctional cytokine, which regulates immune responses, hematopoiesis, acute phase responses, and inflammatory reactions.
MACS GMP Recombinant Human IL-6 is designed for ex vivo cell culture processing. No animal- or human-derived materials were used for manufacture of this product. The product is lyophilized without carrier protein or preservatives.

Disclaimer

MACS GMP Products are for research use and ex vivo cell culture processing only, and are not intended for human in vivo applications. For regulatory status in the USA, please contact your local representative.

Details

Background information

IL-6 is mainly produced by monocytes and macrophages, but as well by fibroblasts or endothelial cells. IL-6 is one of the most important mediators of fever and of the acute phase response. IL-6 induces the terminal maturation of a tivated B cells into plasma cells, and in combination with other factors like IL-2 and IFN-γ it also affects cytotoxic T lymphocytes.

Applications

MACS GMP Recombinant Human IL-6 can be used for a variety of applications, including the ex vivo generation of human dendritic cells from enriched CD14+ monocytes.2–4

Biological activity

The specific activity is determined by proliferation assay according to Gaines-Das and Poole1 using B9 hybridoma cells provided by the German Resource Centre for Biological Material (DSMZ). The proliferation assay was calibrated with the international standard for human IL-6 (NIBSC code 89/548) provided by the National Institute for Biological Standards and Control.
Specific activity: ≥ 8×107 IU/mg

Quality statement

MACS GMP Products are manufactured and tested under a quality management system (ISO 13485) and are in compliance with relevant GMP guidelines. They are designed following the recommendations of USP <1043> on ancillary materials.

Gallery

Figure 1

Figure 1
Mass spectrometry analysis (ESI-MS) of MACS GMP Recombinant Human IL-6. The peak corresponds to the calculated molecular mass of 20812 Da.
Mass spectrometry analysis (ESI-MS) of MACS GMP Recombinant Human IL-6. The peak corresponds to the calculated molecular mass of 20812 Da.

Figure 2

Figure 2
MACS GMP Recombinant Human IL-6 biological activity. Activity of Human IL-6, GMP grade, (blue bar) was compared to another commercially available product (black bar).
MACS GMP Recombinant Human IL-6 biological activity. Activity of Human IL-6, GMP grade, (blue bar) was compared to another commercially available product (black bar).

Library

Selected references

  1. Gaines-Das, R.E. and Poole, S. (1993) The international standard for interleukin-6. Evaluation in an international collaborative study. J. Immunol. Methods 160: 147–153.
  2. Bender, A. et al. (1996) Improved methods for the generation of dendritic cells from nonproliferating progenitors in human blood. J. Immunol. Methods 196: 121–135.
  3. Romani, N. et al. (1996) Generation of mature dendritic cells from human blood. An improved method with special regard to clinical applicability. J. Immunol. Methods 196: 137–151.
  4. Jonuleit et al. (1997) Pro-inflammatory cytokines and prostaglandins induce maturation of potent immunostimulatory dendritic cells under fetal calf serum-free conditions. Eur. J. Immunol. 27: 3135–3142.

Certificates

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Scientific posters

Product Order no. Price

MACS GMP Recombinant Human IL-6

Regulatory status: For research use and ex vivo cell processing only
Availability: worldwide (1)
Source: E. coli
Content: 10 µg

Availability

(1)For availability in your country please contact your local representative.
 
Data sheet
170-076-160 price on request here

MACS GMP Recombinant Human IL-6

Regulatory status: For research use and ex vivo cell processing only
Availability: worldwide (1)
Source: E. coli
Content: 50 µg

Availability

(1)For availability in your country please contact your local representative.
 
Data sheet
170-076-161 price on request here

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Phone: +1 800 FOR MACS
Fax:+1 877 591 1060
macs@miltenyibiotec.com
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