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| Mouse IL-5 Secretion Assay – Cell Enrichment and Detection Kit |
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| Description |
| The Mouse IL-5 Secretion Assay - Cell Enrichment and Detection Kit was developed for the sensitive detection as well as the enrichment of murine IL-5-secreting cells. |
| Details |
Background information
Interleukin-5 (IL-5) is predominantly secreted by CD4+ T cells. It promotes growth and activation of eosinophils, is involved in a range of allergic reactions, and mediates immune reactions against parasites. IL-5 also acts on other cell types such as B cells.
Downstream applications
The Mouse IL-5 Secretion Assays can be used to detect and isolate murine antigen-specific, IL-5-secreting T cells after stimulation with antigen (fig. 1). The assays allow studies on TH2 development and commitment as well as on the mechanism of IL-5 expression. |
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| Figure 1 |
BALB/c mice were immunized and the spleen cells restimulated with keyhole limpet hemocyanin (KLH) as described for the Mouse IFN-γ Secretion Assay. The responding cells were stained and enriched according to secretion of IL-5 using the Mouse IL-5 Secretion Assay - Cell Enrichment and Detection Kit.
In the stimulated sample, 233 IL-5-secreting CD4+ T cells were enriched per 106 CD4+ T cells using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, only six IL-5-secreting CD4+ T cells were detected per 106 CD4+ T cells after enrichment. |
| Stimulated sample |
| A: Before enrichment |
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| B: After enrichment |
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| Unstimulated control |
| C: Before enrichment |
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| D: After enrichment |
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* Percentage represents frequency among CD4+ T cells.
** Percentage represents frequency among enriched cells. |
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| Details |
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| Products |
| Mouse IL-5 Secretion Assay – Cell Enrichment and Detection Kit (PE) |
- for 50 tests with 107 total cells
Download datasheet
130-091-175
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