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| Mouse IL-17 Secretion Assay – Cell Enrichment and Detection Kit |
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| Description |
| The Mouse IL-17 Secretion Assay - Cell Enrichment and Detection Kit was developed for the sensitive detection as well as the enrichment of mouse IL-17A–secreting cells. |
| Details |
Background information
Interleukin 17 (IL-17) is a family of cytokines that play a central role in adaptive immunity as well as autoinflammatory disorders.1 Data from several mouse models suggest that IL-17 plays a key role in the host defense against certain extracellular bacterial infections.
Recently, a new mouse T helper cell subset has been identified, which is dinstinct from Th1 and Th2 cells: IL-17–producing CD4+ T helper (Th17) cells. Th17 cells preferentially produce IL-17A, IL-17F, IL-21, and IL-22. Receptors for IL-17 and IL-22 are expressed on various epithelial tissues, thus Th17 cells are crucial for the cross-talk between immune system and tissues.2 It is now established that Th17 cells are responsible for driving autoimmune inflammation.
After stimulation of mouse cells, IL-17 is not only secreted by CD4+ T cells, but also by CD8+ T cells, γδ T cells, NK cells, and granulocytes. |
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| Figure 2 |
| Mouse splenocytes were stimulated for 3 hours with ionomycin (1 μg/mL) and PMA (10 ng/mL) or left untreated. Cells were enriched by using the Mouse IL-17 Secretion Assay – Cell Enrichment and Detection Kit (PE). T helper cells were counterstained by using CD4-APC. B cells and dead cells were excluded from the analysis. |
| Stimulated sample |
| A: Before enrichment |
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| B: After enrichment |
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| Unstimulated control |
| A: Before enrichment |
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| B: After enrichment |
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| Details |
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| Products |
| Mouse IL-17 Secretion Assay – Cell Enrichment and Detection Kit (PE) |
- for 50 tests with 107 total cells.
Download datasheet
130-094-213
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| References |
| 1. Weaver et al. (2007) Annu. Rev. Immunol. 25: 821–852. |
| 2. Ouyang et al. (2008) Immunity 28: 454–467. |
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