MACS® Supplement B27 PLUS ( #130-093-566 #130-097-263 )

MACS® Supplement B27 PLUS

Overview

MACS® Supplements B27 PLUS are serum-free neural supplements designed for use in combination with MACS Neuro Medium for the in vitro propagation of mature neural cells of the central and peripheral nervous system, or neural stem cells of primary or ES/iPS cell origin.

Details

MACS® Supplement B27 PLUS is a re-optimized formulation of the B27 components designed to improve performance¹. It has been developed for the in vitro propagation of neural cells of the central and peripheral nervous system.

MACS Supplement B27 PLUS has been designed for

optimal growth and long term viability of neural cells of the central and peripheral nervous system
the growth of nearly pure populations of neural cells without the need of an astrocyte feeder layer

MACS® Supplement B27 PLUS w/o Vitamin A is identical to MACS® Supplement B27 PLUS, but without the addition of Vitamin A or other retinoid related components. Vitamin A induces the differentiation of neural stem cells into mature neural cells. MACS® Supplement B27 PLUS w/o Vitamin A is suitable for use with studies of neural stem cells, neural development and neural cell differentiation.

MACS® Supplement B27 PLUS w/o Vitamin A has been designed for use with

primary neural stem or progenitor cells
primary neurospheres
ES/iPS cell derived neural stem cells and neurospheres

Figure 1

Light microscopic picture of neurosphere formation after magnetic cell sorting using Anti-Prominin-1 MicroBeads after 7 days of cultivation in MACS Neuro Medium supplemented with B27 PLUS supplement. Cells were prepared from CD1 mouse brain (P3) using the Neural Tissue Dissociation
Kit (P).

Prominin-1–positive neurospheres

Figure 2

Light microscopic picture of PSA-NCAM–positive neuronal precursor cells.
After 7 days of cultivation in MACS Neuro Medium supplemented with B27 PLUS supplement. PSA-NCAM–positive cells were processed for immunofluorescent detection of anti-GAD67 antibody and goat anti mouse AF-594 (red), a protein marker for neuronal precursor cells. Cells were prepared from CD1 mouse brain (P3) using the Neural Tissue Dissociation Kit (T).

PSA-NCAM–positive neuronal precursor cells

Figure 3

Light microscopic picture of A2B5-positive glial precurosr cells.
After 7 days of cultivation in MACS Neuro Medium supplemented with B27 PLUS supplement. A2B5-positive cells were processed for
immunofluorescent detection of anti-GFAP antibody and goat anti rabbit AF-488 (green), a protein marker for glial precursor cells.
Cells were prepared from CD1 mouse brain (P3) using the Neural Tissue Dissociation Kit (T).

A2B5-positive glial precursor cells

Light microscopic picture of A2B5-positive glial precurosr cells.<br>After 7 days of cultivation in MACS Neuro Medium supplemented with B27 PLUS supplement. A2B5-positive cells were processed for<br>immunofluorescent detection of anti-GFAP antibody and goat anti rabbit AF-488 (green), a protein marker for glial precursor cells.<br>Cells were prepared from CD1 mouse brain (P3) using the Neural Tissue Dissociation Kit (T).