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| Overview |
| Anti-Prominin-1 antibodies can be used to identify and enumerate prominin-1+ cells by flow cytometry or fluorescence microscopy. This antibody is suitable for MACS ® Control applications. |
| Details |
Background information
Clone MB9-3G8 specifically recognizes the mouse Prominin-1 antigen, a glycoprotein with close structural similarity to its human ortholog, CD133. Prominin-1 is expressed on neural stem cells, for example, in the white matter of the cerebellum1, on neuroepithelial tissue cells, and on various other epithelial cells in the mouse embryo2. 5–10% of the neural cells found in the subventricular zone of postnatal mice have been shown to be prominin-1-positive. In the adult mouse, prominin-1 has been detected in the brain ependymal layer2, and in kidney tubules2,3. |
| Clone | Isotype |
| MB9-3G8 | Rat lgG1 |
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| Figure 1 |
| Mouse brain cells (postnatal day 1) were dissociated using the Neural Tissue Dissociation Kit (P) and stained with Anti-Prominin-1-APC. Cells were analyzed by flow cytometry. |
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| Products |
| Anti-Prominin-1-PE, mouse |
- for 100 tests (1)
Download datasheet
130-092-334
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| Anti-Prominin-1-APC, mouse |
- for 100 tests (1)
Download datasheet
130-092-335
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| Anti-Prominin-1-Biotin, mouse |
- for 100 tests (2)
Download datasheet
130-092-441
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| Anti-Prominin-1 pure, mouse |
- 100 μg in 1 mL
Download datasheet
130-092-442
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| (1) One test corresponds to fluorescent labeling of up to 107 cells in a total volume of 100 μL. |
| (2) One test corresponds to labeling of up to 107 cells in a total volume of 100 μL. |
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| References |
| 1. Lee et al. (2005) Nat. Neurosci. 8: 723–729. |
| 2. Weigmann et al. (1997) Proc. Natl. Acad. Sci. USA 94: 12425–12430. |
| 3. Corbeil et al. (2001) Traffic 2: 82–91. |
| 4. Singh et al. (2003) Cancer Res. 63: 5821–5828. |
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